K2129-100 assays | Lactose Colorimetric/Fluorometric Assay Kit Clinisciences

Background

Lactose (C₁₂H₂₂O₁₁) is a disaccharide sugar consisting of one galactose and one glucose. Lactose makes up around 2-8% of milk. Some people, particularly infants, lack lactase to digest lactose leading to lactose accumulation in blood (Galactosemia), which then causes renal failure, enlarged liver, cataracts and brain damage.

The Lactose Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for detection of lactose levels in various biological samples (plasma, serum, other body fluids, growth media, food, etc.) based on colorimetric and fluorometric method. In the assay, lactose is hydrolyzed to glucose and galactose, which is then oxidized producing fluorescence (Ex/Em 535/587 nm) and color (O.D 570 nm). Free galactose can be corrected by a background control in the absence of lactase. The assay is performed without pretreatment of samples. The kit is suited for a high throughput assay.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 1 hour; Kit contains all necessary reagents for accurate measurement of lactose.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Lactose Assay Buffer, Probe (DMSO solution), Lactase (Lyophilized), Lactose Enzyme Mix (Lyophilized), HRP (Lyophilized), Lactose Standard (100 nmol/µL)

Lactose Colorimetric/Fluorometric Assay Kit

Référence K2129-100 assays

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