MBP, Recombinant, E. coli, aa27-396 (Maltose Binding Protein)

Référence 369097-500ug

Conditionnement : 500ug

Marque : US Biological

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369097 MBP, Recombinant, E. coli, aa27-396 (Maltose Binding Protein)

Clone Type
Polyclonal
Grade
Highly Purified
Accession #
NP_418458
Shipping Temp
Blue Ice
Storage Temp
-20°C

MBP, also as known as Maltose Binding Protein, is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. ||Source:|Recombinant protein corresponding to aa27-396 E. coli MBP expressed in E. coli.||Molecular Weight: |~40.8 kD (371aa)||Amino Acid Sequence:|MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTRIT K||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: Recombinant, E. coli|Purity: ≥95% (SDS-PAGE)|Concentration: ~1mg/ml (determined by Absorbance at 280nm)|Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Purity
≥95% (SDS-PAGE)
References
1. Fox JD., et al. (2001) Protein Sci. 10(3): 622-30. |2. Riggs P., et al. (2000) Mol. Biotechnol. 15(1): 51-63.