Mouse Anti-Chicken CD45-PE
Référence 8270-09
Conditionnement : 0.1mg
Marque : Southern Biotech
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| Clone | LT40 |
|---|---|
| Isotype | Mouse (BALB/c) IgMκ |
| Isotype Control | Mouse IgM-PE (11E10) |
| Specificity | Chicken CD45 |
| Description | Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. Levels of CD45 expression detected by the monoclonal antibody LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45low) of thymocytes. The CD45low population contains exclusively thymocytes with the CD3-CD4-CD8low phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8low to CD4+CD8+ and before the expression of CD3. The ability of the monoclonal antibody LT40 to discriminate this CD45low subpopulation provides a suitable marker for the CD3-CD4-CD8low immediate precursors to the CD3-CD4+CD8+ thymocytes. |
| Immunogen | Chicken bursal lymphocytes |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications | Flow Cytometry – Quality tested 1,6-18 Immunohistochemistry-Frozen Sections – Reported in literature 2 Immunohistochemistry-Paraffin Sections – Reported in literature 3 Immunohistochemistry-Whole Mount – Reported in literature 4 Immunocytochemistry – Reported in literature 19 Immunoprecipitation – Reported in literature 1 Separation – Reported in literature 5 |
| RRID Number | AB_2796474 |
| Gene ID | 386580 (Chicken) |
| Gene ID Symbol | PTPRC (Chicken) |
Documentation
Technical Bulletin Safety Datasheet
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- 2. Corbel C. Expression of αVβ3 integrin in the chick embryo aortic endothelium. Int J Dev Biol. 2002;46:827-30. (IHC-FS)
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