Mouse Anti-Human CD20-AF700
Référence 9350-27
Conditionnement : 100tests
Marque : Southern Biotech
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| Clone | B-Ly1 |
|---|---|
| Isotype | Mouse IgG1κ |
| Isotype Control | Mouse IgG1-AF700 (15H6) |
| Specificity | Human CD20 |
| Alternative Names | Leu-16, B1, Bp35, MS4A1, S7 |
| Description | CD20 is a 33-37 kDa unglycosylated phosphoprotein. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. It is expressed on all stages of B cell differentiation except plasma cells. The CD20 molecule is involved in regulation of B cell differentiation presumably via its reported function as a Ca++ channel subunit. |
| Conjugate | AF700 (Alexa Fluor® 700) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 5,6,8,9 Immunohistochemistry-Frozen Sections – Reported in literature 1,2 Immunohistochemistry-Paraffin Sections – Reported in literature 3 Immunoprecipitation – Reported in literature 4 Blocking – Reported in literature 5 Separation – Reported in literature 6 Cytoxicity – Reported in literature 7 |
| RRID Number | AB_2796769 |
| Gene ID | 931 (Human) |
| Gene ID Symbol | MS4A1 (Human) |
| Gene ID Aliases | B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16 |
| UniProt ID | P11836 (Human |
| UniProt Name | CD20_HUMAN (Human) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 1. Kang YM, Zhang X, Wagner UG, Yang H, Beckenbaugh RD, Kurtin PJ, et al. CD8 T cells are required for the formation of ectopic germinal centers in rheumatoid synovitis. J Exp Med. 2002;195:1325-36. (IHC-FS)
- 2. Yoon S, Zhang X, Lee IY, Spencer N, Vo P, Choi YS. CD9 is a novel marker for plasma cell precursors in human germinal centers. Biochem Biophys Res Commun. 2013;431:41-6. (IHC-FS)
- 3. Yang M, Xu Z, Zhuang Z. Macrophages affect immune inflammation and proliferation in benign prostatic hyperplasia via androgen receptor and CD40/CD40L signaling pathway. Tissue Cell. 2020;64:101343. (IHC-PS)
- 4. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD20. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al, editors. Leukocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989. p. 46-48. (IP)
- 5. Tedder TF, Penta A. Structure of the CD20 antigen and gene of human and mouse B-cells: use of transfected cell lines to examine the workshop panel of antibodies. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al, editors. Leukocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989. p. 48-50. (FC, Block)
- 6. Pawelec G, Müller R, Rehbein A, Hähnel K, Ziegler BL. Extrathymic T cell differentiation in vitro from human CD34+ stem cells. J Leukoc Biol. 1998;64:733-9. (FC, Sep)
- 7. Mössner E, Brünker P, Moser S, Püntener U, Schmidt C, Herter S, et al. Increasing the efficacy of CD20 antibody therapy through the engineering of a new type II anti-CD20 antibody with enhanced direct and immune effector cell-mediated B-cell cytotoxicity. Blood. 2010;115:4393-402. (Cyto)
- 8. Odendahl M, Jacobi A, Hansen A, Feist E, Hiepe F, Burmester GR, et al. Disturbed peripheral B lymphocyte homeostasis in systemic lupus erythematosus. J Immunol. 2000;165:5970-9. (FC)
- 9. Odendahl M, Keitzer R, Wahn U, Hiepe F, Radbruch A, Dörner T, et al. Perturbations of peripheral B lymphocyte homoeostasis in children with systemic lupus erythematosus. Ann Rheum Dis. 2003;62:851-8. (FC)