Mouse Anti-Human IgE Fc-AP

Référence 9250-04

Conditionnement : 1.0mL

Marque : Southern Biotech

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More Information
Clone HP6029
Isotype Mouse IgG2aκ
Isotype Control Mouse IgG2a-AP (HOPC-1)
Specificity Human IgE Fc
Immunogen PS human IgE myeloma protein
Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 1-8,11
FLISA – Quality tested
ELISpot – Reported in literature 5
Western Blot – Reported in literature 9,10
Purification – Reported in literature 11


RRID Number AB_2796718
Gene ID 3497 (Human)
Gene ID Symbol IGHE (Human)
Gene ID Aliases IgE
UniProt ID P01854 (Human
UniProt Name IGHE_HUMAN (Human)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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  1. 1. Agea E, Russano A, Bistoni O, Mannucci R, Nicoletti I, Corazzi L, et al. Human CD1-restricted T cell recognition of lipids from pollens. J Exp Med. 2005;202:295-308. (ELISA)
  2. 2. Milovanovic M, Heine G, Zuberbier T, Worm M. Allergen extract-induced interleukin-10 in human memory B cells inhibits immunoglobulin E production. Clin Exp Allergy. 2009;39:671-8. (ELISA)
  3. 3. Heine G, Dahten A, Hilt K, Ernst D, Milovanovic M, Hartmann B, et al. Liver X receptors control IgE expression in B cells. J Immunol. 2009;182:5276-82. (ELISA)
  4. 4. Milovanovic M, Drozdenko G, Weise C, Babina M, Worm M. Interleukin-17A promotes IgE production in human B cells. J Invest Dermatol. 2010;130:2621-8. (ELISA)
  5. 5. Weise C, Hilt K, Milovanovic M, Ernst D, Rühl R, Worm M. Inhibition of IgE production by docosahexaenoic acid is mediated by direct interference with STAT6 and NFκB pathway in human B cells. J Nutr Biochem. 2011;22:269-75. (ELISA, ELISPOT)
  6. 6. Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)
  7. 7. Qian Y, Jeong JS, Abdeladhim M, Valenzuela JG, Aoki V, Hans-Filhio G, et al. IgE anti-LJM11 sand fly salivary antigen may herald the onset of Fogo selvagem in endemic Brazilian regions. J Invest Dermatol. 2015;135:913-5. (ELISA)
  8. 8. Bundhoo A, Paveglio S, Rafti E, Dhongade A, Blumberg RS, Matson AP. Evidence that FcRn mediates the transplacental passage of maternal IgE in the form of IgG anti-IgE/IgE immune complexes. Clin Exp Allergy. 2015 Feb 5. doi: 10.1111/cea.12508. [Epub ahead of print]. (ELISA)
  9. 9. Martos G, Pineda-Vadillo C, Miralles B, Alonso-Lebrero E, López-Fandiño R, Molina E, et al. Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion. J Agric Food Chem. 2012;60:5215-20. (WB)
  10. 10. Benedé S, López-Expósito I, Giménez G, Grishina G, Bardina L, Sampson HA, et al. In vitro digestibility of bovine β-casein with simulated and human oral and gastrointestinal fluids. Identification and IgE-reactivity of the resultant peptides. Food Chem. 2014;143:514-21. (WB)
  11. 11. Kleine-Tebbe J, Hamilton RG, Roebber M, Lichtenstein LM, MacDonald SM. Purification of immunoglobulin E (IgE) antibodies from sera with high IgE titers. J Immunol Methods. 1995;179:153-64. (Purification, ELISA)

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