Mouse IgG3, Human ads Antibody : APC/CY7

Référence OASB01636

Conditionnement : 0.25mg

Marque : Aviva Systems Biology

Demander plus d'informations

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Goat Anti-Mouse IgG3 Antibody - APC/CY7 Conjugated (OASB01636)

Datasheets/ManualsPrintable datasheet for Goat Anti-Mouse IgG3 Antibody - APC/CY7 Conjugated (OASB01636)
Product Info
Tested Species ReactivityMouse
Predicted Species ReactivityMouse
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationAPC-Cyanine 7
ApplicationFC
Additional InformationDescription: Pooled antisera from goats hyperimmunized with mouse IgG3; purified by affinity chromatography on mouse IgG3 covalently linked to agarose; reacts with the heavy chain of mouse IgG3; cross-adsorbed against mouse IgG1, IgG2a, IgG2b, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity
Reconstitution and StorageStore at 2-8C
ImmunogenMouse IgG3
PurificationAffinity chromatography on mouse IgG3 covalently linked to agarose.
Concentration0.25 mg/mL
SpecificityIgG3
CharacterizationTo insure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent.
WarningReagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionImmunofluorescence:
ELISA:
FITC, TRITC, TXRD BIOT, and CY5 conjugates
R-PE, SPRD, R-PE- CY7, and APC-CY7 conjugates
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Application InfoIndirect immunostaining in conjunction with mouse IgG3 monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunoblotting
Other Applications DataSince applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates aresupplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD) conjugate is supplied as 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- R- phycoerythrin- Cyanine 7 (R- PE- CY 7) and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates aresupplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross AbsorptionMouse IgM, IgG1, IgG2a, IgG2b and IgA, pooled human sera and purified humanparaproteins.
Gene SymbolIGHG3
Gene Full NameIg gamma-3 chain C region
Alias SymbolsIgG, IgG3, AI3240
NCBI Gene Id380795
Uniprot IDP03987