Mouse IL-12p70 ELISA Kit (96-well)
Référence EA100130
Conditionnement : 1x96wells
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Mouse IL-12p70 ELISA Kit (96-well)
Product Data | |
ACCN | NM_008352 |
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Assay Length | 3 hour |
Assay Platform | 96-well plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Type | Sandwich |
Background | Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of a human B-lymphoblastoid cell line . Biologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. While the p40 and p35 subunits by themselves do not have IL-12 activity, the p40 homodimer has been shown to bind the IL-12 receptor and is an IL-12 antagonist . Mature mouse p35 subunit is composed of 193 amino acid (aa) residues and contains seven cysteines plus one potential N-linked glycosylation site . Mature mouse p40 subunit has 313 aa, with 13 cysteines and five potential N-linked glycosylation sites . Mouse p35 and p40 subunits show 63% and 72% aa identity, respectively, to the human p35 and p40 subunits . Although mouse IL-12 is active on both human and mouse cells, human IL-12 is only active on human cells. IL-12 has been shown to have multiple effects on T lymphocytes and natural killer (NK) cells. Some of these effects include the induction of IFN-. and TNF production by T and NK cells, the enhancement of cytotoxic activity of T and NK cells and the stimulation of T and NK cell proliferation. IL-12 has also been shown to be a central mediator of the cell-mediated immune response by promoting Th1 development . Cell surface staining for IL-12 on a human monocytic and a mouse macrophage cell line has been reported, suggesting that membrane-associated IL-12 may exist . Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Although a human B cell line has been shown to produce IL-12, fresh B cells are apparently not producers of IL-12. |
Components |
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Curve Range | 0.02 - 1.0 ng/mL |
figure_legend | Representative standard curve for IL-12p70 ELISA. IL-12p70 was diluted in serial two-fold steps in Sample Diluent. |
Format | 96-well strip plate |
Gene Symbol | Il12b |
Interference | No significant interference observed with available related molecules. |
Locus ID | 16160 |
Reactivity | Mouse |
Reactivity | Mouse |
Reactivity | Mouse |
Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
Sample Volume | 20 uL |
Sensitivity | 7pg/mL |
Signal | Colorimetric |
Species | Mouse |
Specificity | Natural and recombinant Mouse IL-12p70 Ligand |
Standard Curve | /assets/images/elisa/kits/128/cme0013-096-1-e.jpg |
Target Symbol | Il12b |
New | No |
Description | Mouse IL-12p70 ELISA Kit (96-well) |