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Mouse Monoclonal Anti-MUC2 (Mucin 2) [Clone SPM513]
Référence NB-36-00527XX-P1
Conditionnement : 100ug
Marque : Neo Biotech
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MUC2 (Mucin 2) Antibody [SPM513]
Catalog Number Formulation Size Price
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human colon stained with MUC2 Mouse Monoclonal Antibody (SPM513).
SDS-PAGE Analysis of Purified MUC2 Mouse Monoclonal Antibody (SPM513). Confirmation of Integrity and Purity of Antibody
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro). Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
MUC2
Positive Control
LS174T cells. Human small intestine or colon tissue.
Immunogen
A synthetic peptide of 29 amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH.
Alternate Names
Mucin-2, Intestinal mucin-2, Mucin-2, Intestinal mucin-2, Mucin like protein (MLP), SMUC
Functions
- Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
Key References
- Xing PX, et al. Second-generation monoclonal antibodies to intestinal MUC2 peptide reactive with colon cancer. J Natl Cancer Inst. 1992; 84(9):699-703.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/8300571/
- https://pubmed.ncbi.nlm.nih.gov/1400449/
- https://pubmed.ncbi.nlm.nih.gov/1885763/
- https://pubmed.ncbi.nlm.nih.gov/2703501/
- https://pubmed.ncbi.nlm.nih.gov/1550588/
- https://pubmed.ncbi.nlm.nih.gov/11445551/
- https://pubmed.ncbi.nlm.nih.gov/12374796/
- https://pubmed.ncbi.nlm.nih.gov/12582180/
- https://pubmed.ncbi.nlm.nih.gov/18669648/
- https://pubmed.ncbi.nlm.nih.gov/18327567/
- https://pubmed.ncbi.nlm.nih.gov/19359471/
- https://pubmed.ncbi.nlm.nih.gov/19432394/
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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