NB-4
Référence 300299
Conditionnement : 1cryovial
Marque : CLS Cell Lines Service
NB-4 Cells
Introduction to the NB-4 cell line
Description | NB-4 cells are a human acute promyelocytic leukemia (APL) cell line established from the bone marrow of a patient experiencing the second relapse of acute promyelocytic leukemia. This cell line is characterized by the presence of the t(15;17) chromosomal translocation, which results in the PML-RARα fusion gene, a hallmark of APL. The NB4 cell line serves as a pivotal model for studying the pathogenesis of APL and the mechanisms of action of therapeutic agents inducing differentiation such as retinoic acid (ATRA) and arsenic trioxide (ATO). As a promyelocytic leukemia cell line, NB-4 cells exhibit an aberrant pattern of differentiation that is characteristic of APL. This aberrancy provides a unique window into the cellular mechanisms underlying leukemia progression and the potential for therapeutic intervention. The ability of NB-4 cells to undergo apoptosis, or programmed cell death, upon exposure to certain chemotherapeutic agents or differentiation inducers like retinoic acid, makes them an invaluable tool for studying cell apoptosis in the context of leukemia. The NB-4 cell line also demonstrates bilineage potential, highlighting its ability to differentiate along multiple hematopoietic lineages under specific conditions. In conclusion, the NB-4 cell line, with its unique properties and responsiveness to differentiation inducers like retinoic acid, continues to be a pivotal resource for researchers delving into the intricacies of promyelocytic leukemia and the broader field of oncology. |
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Organism | Human |
Tissue | Bone marrow |
Disease | Acute promyelocytic leukemia |
Synonyms | NB4, NB.4 |
Characteristics
Age | 23 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | B lymphocyte |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | NB-4 (Cytion catalog number 300299) |
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Biosafety level | 1 |
Genetic makeup of the promyelocytic leukemia NB4 cell line
Antigen expression | CD4+, CD14-, CD36- |
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Reverse transcriptase | Negative |
Karyotype | t(15,17) (q22,q11-12) translocation |
Culturing guidelines
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 35 to 40 hours |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality assurance of NB4 cells
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 11,12 D13S317: 11,12 D16S539: 9 D5S818: 13 D7S820: 10,13 TH01: 7,9.3 TPOX: 8,11 vWA: 16,19 D3S1358: 15,17 D21S11: 28,33.2 D18S51: 12,14 Penta E: 7,13 Penta D: 10,13 D8S1179: 10,14 FGA: 21,22 |
HLA alleles | A*: 11:01:01 B*: 01.01.1900 11:01, 01.01.1900 16:01 C*: 03:04:01, 04:01:01 DRB1*: 01:01:01, 04:04:01 DQA1*: 01:01, 03:01:01 DQB1*: 03:02, 05:01:01 DPB1*: 01:01:01, 04:01:01 E: 01:01:01 |