Nestin antibody [GT623]
Référence GTX630201-25ul
Conditionnement : 25ul
Marque : Genetex
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
![Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | GT623 |
Isotype | IgG1 |
Application | WB, ICC/IF |
Reactivity | Human |
| Package | 100 μl, 25 μl |
Datasheet File View product citations for antibody GTX630201 on CiteAb
PRODUCT
Summary
Nestin antibody recognizes nestin, a type-IV intermediate filament protein that migrates at ~240 kDa on western blot. Nestin is an established marker of mesodermal and neuroectodermal fates, and is expressed in neural stem cells. It is also enriched in endothelial cells, regardless of their proliferative state.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:3000 |
| ICC/IF | 1:100-1:1000 |
Not tested in other applications.
Calculated MW
177 kDa. ( Note )
Positive Control
SK-N-SH , IMR32
Product Note
We do not recommend use of this product for Mouse,Rat samples.
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Nestin. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888203
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nestin , Nbla00170
Background
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
Database
DATA IMAGES
| | GTX630201 ICC/IF ImageNestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.Green: Nestin stained by Nestin antibody [GT623] (GTX630201) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920). |
| | GTX630201 WB ImageVarious whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
| | GTX630201 WB Image Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
Application Reference
S Sakatoku et al. Stem Cells Int 2024; : 7685280 Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium
Application : ICC/IF
Seo Y et al. Int J Mol Sci 2023; 24 (6) Endogenous Neural Stem Cell Activation after Low-Intensity Focused Ultrasound-Induced Blood-Brain Barrier Modulation.
Application : WB
Yao P et al. Folia Histochem Cytobiol 2022; (Epub) Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.
Ma L et al. J Radiat Res 2022; (Epub) Irradiated microvascular endothelial cells may induce bystander effects in neural stem cells leading to neurogenesis inhibition.
Ming Zhang et al. Nat Commun 2021; 12 (1) : 5091 Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.
Submit a Reference REVIEW
Application Reference
S Sakatoku et al. Stem Cells Int 2024;:7685280 Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium
Application : ICC/IF
Seo Y et al. Int J Mol Sci 2023; 24 (6) Endogenous Neural Stem Cell Activation after Low-Intensity Focused Ultrasound-Induced Blood-Brain Barrier Modulation.
Application : WB
Yao P et al. Folia Histochem Cytobiol 2022; (Epub) Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.
Ma L et al. J Radiat Res 2022; (Epub) Irradiated microvascular endothelial cells may induce bystander effects in neural stem cells leading to neurogenesis inhibition.
Ming Zhang et al. Nat Commun 2021; 12 (1):5091 Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.
Deng H et al. Cell Transplant 2020; 29:9.6369E+14 Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.
Koffler J et al. Nat Med 2019; 25 (2):263-269 Biomimetic 3D-printed scaffolds for spinal cord injury repair.
Mansel C et al. Neurotoxicol Teratol 2019; 72:58-70 Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.
Liu H et al. Cell Death Differ 2018; (Epub) Biochemical re-programming of human dermal stem cells to neurons by increasing mitochondrial membrane potential.
Reactivity : Human
Xie MQ et al. Neuroscience 2017; 352:64-78 Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model.
Aguilera-Castrejon A et al. Neurochem Res 2016; (Epub) Improved Proliferative Capacity of NP-Like Cells Derived from Human Mesenchymal Stromal Cells and Neuronal Transdifferentiation by Small Molecules.
Reactivity : Human
Pringproa K et al. PLoS One 2015; 10 (8):e0135850 Tropism and Induction of Cytokines in Human Embryonic-Stem Cells-Derived Neural Progenitors upon Inoculation with Highly- Pathogenic Avian H5N1 Influenza Virus.
Reactivity : Human
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