PC-3M
Référence 305061
Conditionnement : 1cryovial
Marque : CLS Cell Lines Service
PC-3M Cells
General information
Organism | Human |
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Tissue | Prostate |
Disease | Prostate carcinoma |
Metastatic site | Bone |
Synonyms | PC3-M, PC-3/M, PC3M, Pc3M |
Characteristics
Age | 62 years |
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Gender | Male |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | PC-3M (Cytion catalog number 305061) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3 (Cytion article number 820608a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 11 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,11 TH01: 6,7 TPOX: 8,9 vWA: 17 D3S1358: 16 D21S11: 29,31.2 D18S51: 14,15 Penta E: 10,17 Penta D: 9 D8S1179: 13 FGA: 24 D6S1043: 14,18 D2S1338: 18,20 D12S391: 21 D19S433: 14 |