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> Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay™

Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay™

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Référence P4071-60-1Kit

Conditionnement : 1Kit

Marque : US Biological

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P4071-60 Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay™

Clone Type
Polyclonal
Shipping Temp
Blue Ice
Storage Temp
4°C

This Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay™ is suitable for detection and quantitation of acid and alkaline phosphatase activity from cell extracts.||Kit Capacity: |100 assays per kit.||Kit Components:|Malachite Green Solution A|Malachite Green Additive|Phosphate Standard|Serine Phosphopeptide (RRApSVA)|pNPP (p-Nitrophenyl Phosphate)|NiCl2, 40mM|pNPP Ser/Thr Assay Buffer|96-well microtiter plate||Quality Control:|The components of this kit have matched to optimize sensitivity and range of detection. Phosphate released is determined by comparing absorbance to standard curve of step 2. Using this kit, 0.5U of calf intestine alkaline phosphatase released 66.9pmol phosphate per minute using the phosphopeptide (RRApSVA).||Note: |Do not perform the Malachite Green Assay using phosphate buffers or with biological samples that contain high levels of free phosphate. Remove free phosphate from samples by desalting column or dialysis.||Storage and Stability: |Store kit at 4°C. Stable for 1 year after receipt.

Applications
Kit Components:|P4071-60A1, Malachite Green (Solution A), 1x20ml|Supplied as a liquid in 10mM ammonium molybdate, 1N HCl, 3.4% ethanol, 0.034% malachite green.|P4071-60A2, Malachite Green Additive (Solution B), 1x 500ul|Supplied as a liquid in 1% Tween®-20.|P4071-60A3, Phosphate Standard (Solution C), 1x1ml|Concentration, 1.0mM, Supplied as a liquid in 1mM potassium phosphate monobasic, KH2PO4.|P4071-60A4, Serine Phosphopeptide (RRApSVA), 1x1mg, Supplied as a lyophilized powder.|P4071-60A5, p-Nitrophenyl Phosphate (pNPP), 2xtablets, Each tablet contains 5mg pNPP.|P4071-60A6, Nickel Chloride (40mM), 1x1ml, Supplied as a liquid.|P4071-60A7, pNPP Ser/Thr Assay Buffer, 1x20ml, Supplied as a liquid in 50mM Tris-HCl, pH 7.0, 0.1mM CaCl2.|P4071-60A8, Microtiter Plate, 1x96wells, 1/2 volume flat bottom plate||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Harder, K.W., et al., Biochem. J. 298: 395-401 (1994). 2. Donella-Deana, A., et al., Biochim. Biophys. Acta 1094: 130-133 (1991). 3. Donella-Deana, A., et al., Biochim. Biophys. Acta 1051: 199-202 (1990). 4. Ek man, P. & Jager, O., Annal. Biochem. 214: 138-141 (1993).