• Specifications

    Product Description

    Rabbit anti-mouse IgG recognizes mouse IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.

    Immunogen

    Mouse IgG whole molecule

    Host

    Rabbit

    Reactivity

    Mouse

    Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgG and mouse serum.

    Form

    Lyophilized

    Conjugation

    FITC

    Purification

    This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Isotype

    IgG

    Conjugation Note

    2.79 moles Fluorescein (FITC) per mole of IgG

    Recommend Usage

    Flow Cytometry
    Fluorescence-linked immunosorbent assay (1:10000-1:50000)
    Immunofluorescence (1:1000-1:5000)
    Western Blot
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% sodium azide).

    Storage Instruction

    Store at 4°C on dry atmosphere prior to restoration.
    After reconstitution with 1.0 mL deionized water (or equivalent), store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunofluorescence

    Flow Cytometry

    Fluorescence-linked Immunosorbent Assay

  • Publication Reference