Rabep1 (NM_019400) Mouse Tagged ORF Clone
Référence MR217501
Conditionnement : 10ug
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| Product Data | |
| Target Symbol | Rabep1 |
|---|---|
| Synonyms | Rab5ep; rabaptin-5; rabaptin-5alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >MR217501 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGCCGGGCCCGGCTCCCCAGCCTGACGTTTCTCTTCAGCAACGGGTAGCAGAATTGGAAAAAA TTAATGCAGAATTTTTACGTGCACAGCAGCAGCTTGAGCAAGAATTTAATCAAAAGAGAGCAAAATTTAA GGAGCTCTATTTGGCTAAAGAGGAGGATCTGAAGAGGCAAAATGCAGTATTGCAAGCTGCACAGGACGAT TTGGGTCACCTTCGGACACAGCTGTGGGAGGCTCAGGCAGAGATGGAGAACATTAAGGCAATTGCCACAG TCTCTGAGAATACTAAGCAAGAAGCTATAGATGAAGTTAAAAGGCAATGGAGAGAAGAAGTTGCTTCACT TCAGGCTATAATGAAAGAGACAGTCCGTGACTATGAGCATCAGTTTCACCTGAGGCTGGAGCAGGAGCGA GCACAGTGGGCACAGTATCGAGAATCTGCAGAGCGGGAAATAGCTGACTTAAGAAGAAGGTTGTCTGAAG GTCAAGAGGAAGAAAATTTAGAAAATGAAATGAAAAAGGCCCAAGAGGATGCTGAAAAGCTTCGTTCTGT CGTGATGCCCATGGAGAAGGAAATTGCAGCTCTGAAAGATAAACTGACAGAAGCTGAAGACAAGATTAAA GAGCTGGAGGCCTCAAAGGTTAAGGAGCTGAATCATTATCTGGAGGCCGAGAAGTCTTGCAGGACTGACC TGGAGATGTACGTAGCTGTTTTGAACACTCAGAAATCTGTGCTACAGGAAGATGCTGAGAAATTGAGGAA AGAACTGCATGAAGTTTGCCATCTCTTGGAACAAGAACGACAACAACACAACCAGTTAAAGCATACATGG CAGAAGGCCAACGACCAGTTTCTGGAATCTCAGCGTTTGCTGATGAGAGACATGCAGAGAATGGAGATTG TGTTAACTTCGGAACAGCTCCGACAAGTTGAAGAACTAAAGAAGAAAGACCAGGAGGAGGATGAACAACA AAGGGTCAATAAGAGAAAAGATAACAAGAAAACAGATACCGAAGAAGAAGTAAAAATACCAGTAGTGTGT GCTTTAACTCAAGAAGAATCTTCAACCCCATTATCAAATGAAGAGGAGCATTTAGACAGCACCCATGGGT CAGTCCATTCCTTGGATGCAGACTTGATGTTACCATCTGGAGATCCATTTAGTAAATCGGACAATGACAT GTTTAAAGATGGACTCAGGAGAGCTCAGTCTACAGACAGCTTGGGAACCTCAAGCTCATTGCAATCCAAA GCTTTAGGCTATAACTACAAAGCGAAATCTGCTGGAAATTTGGATGAGTCGGATTTTGGACCACTGGTTG GAGCAGATTCTGTGTCTGAGAACTTTGATACTGTGTCCCTTGGGTCACTGCAGATGCCAAGTGGATTTAT GTTAACCAAAGATCAAGAAAGAGCAATTAAGGCAATGACACCTGAGCAGGAGGAGACAGCATCCCTCCTC TCCAGTGTCACCCAGGGTATGGAGAGTGCATATGTATCTCCCAGTGGTTACCGCTTAGTGAGTGAGACGG AATGGAATCTCCTGCAGAAAGAGGTACATAATGCTGGAAATAAGCTTGGTAGACGTTGTGATATGTGTTC CAATTATGAAAAACAGTTACAAGGAATTCAGATTCAGGAAGCTGAAACAAGAGACCAGGTGAAAAAACTG CAGTTAATGCTCAGGCAAGCTAATGACCAGCTTGAGAAGACGATGAAAGAGAAGCAGGAGCTGGAGGACT TCCTCAAGCAGAGTGCGGAGGACTCAAGCCACCAGATATCTGCACTTGTTTTAAGAGCCCAAGCCTCTGA GGTCTTACTTGAAGAGTTACAACAGAGCTTTTCCCAAGCAAAGAGGGACGTTCAGGAGCAGATGGCGGTG CTGATGCAGTCACGTGAACAGGTTTCAGAAGAGTTGGTGAGGTTACAGAAAGACAACGACAGCCTCCAGG GGAAGCACAGCTTGCATGTGTCATTACAGCTAGCAGAAGACTTCATTCTTCCAGACACTGTGGAGGTGCT CCGGGAACTGGTTTTAAAATATCGTGAGAACATTGTTCATGTGCGCACAGCAGCAGACCACATGGAAGAG AAGCTGAAGGCGGAAATACTTTTCCTAAAAGAGCAAATTCAAGCGGAACAGTGTTTAAAAGAAAACCTGG AAGAGACACTACAGCTGGAAATAGAAAACTGCAAGGAGGAAATAGTCCTAGCTTCCATTTCTAGTCTAAA AGCTGAATTAGAAAGAATAAAGGTTGAAAAGGGACAGTTGGAATCTACATTAAGAGAGAAGTCGCAACAA CTTGAGAGTCTCCAAGAAATGAAAGTTAATTTAGAGGAACAGTTAAAGAAAGAAACTGCTGCTAAGGCTA CTGTTGAACAGCTCATGTTTGAAGAGAAGAACAAAGCTCAGAGGTTGCAGACAGAATTAGATGTCAGTGA ACAAGTACAGAGAGATTTTGTAAAACTTTCTCAGACTCTTCAGGTGCAGTTGGAGAGGATCCGGCAAGCA GACTCCTTGGAGAGGATCCGGGCTATTCTGAATGATACCAAACTGACAGACATTAACCAGCTCCCTGAGA CA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >MR217501 protein sequence Red=Cloning site Green=Tags(s) MAQPGPAPQPDVSLQQRVAELEKINAEFLRAQQQLEQEFNQKRAKFKELYLAKEEDLKRQNAVLQAAQDD LGHLRTQLWEAQAEMENIKAIATVSENTKQEAIDEVKRQWREEVASLQAIMKETVRDYEHQFHLRLEQER AQWAQYRESAEREIADLRRRLSEGQEEENLENEMKKAQEDAEKLRSVVMPMEKEIAALKDKLTEAEDKIK ELEASKVKELNHYLEAEKSCRTDLEMYVAVLNTQKSVLQEDAEKLRKELHEVCHLLEQERQQHNQLKHTW QKANDQFLESQRLLMRDMQRMEIVLTSEQLRQVEELKKKDQEEDEQQRVNKRKDNKKTDTEEEVKIPVVC ALTQEESSTPLSNEEEHLDSTHGSVHSLDADLMLPSGDPFSKSDNDMFKDGLRRAQSTDSLGTSSSLQSK ALGYNYKAKSAGNLDESDFGPLVGADSVSENFDTVSLGSLQMPSGFMLTKDQERAIKAMTPEQEETASLL SSVTQGMESAYVSPSGYRLVSETEWNLLQKEVHNAGNKLGRRCDMCSNYEKQLQGIQIQEAETRDQVKKL QLMLRQANDQLEKTMKEKQELEDFLKQSAEDSSHQISALVLRAQASEVLLEELQQSFSQAKRDVQEQMAV LMQSREQVSEELVRLQKDNDSLQGKHSLHVSLQLAEDFILPDTVEVLRELVLKYRENIVHVRTAADHMEE KLKAEILFLKEQIQAEQCLKENLEETLQLEIENCKEEIVLASISSLKAELERIKVEKGQLESTLREKSQQ LESLQEMKVNLEEQLKKETAAKATVEQLMFEEKNKAQRLQTELDVSEQVQRDFVKLSQTLQVQLERIRQA DSLERIRAILNDTKLTDINQLPET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_019400 |
| ORF Size | 2595 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019400.1, NM_019400.2, NM_019400.3, NP_062273.2 |
| RefSeq Size | 5440 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 54189 |
| UniProt ID | O35551 |
| Cytogenetics | 11 43.21 cM |
| MW | 99.7 kDa |
| Summary | Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium (PubMed:25405894).[UniProtKB/Swiss-Prot Function] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| MC222262 | Rabep1 (untagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1), (10ug) | 10 ug | | |
| MG217501 | Rabep1 (tGFP-tagged) - Mouse rabaptin RAB GTPase binding effector protein 1 (Rabep1), (10ug) | 10 ug | | |
| MR217501L3 | Lenti ORF clone of Rabep1 (Myc-DDK-tagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1) | 10 ug | | |
| MR217501L4 | Lenti ORF clone of Rabep1 (mGFP-tagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.