Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Rat Insulin ELISA Kit

Rat Insulin ELISA Kit

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Référence CC-90010

Conditionnement : 96tests

Marque : Crystal Chem

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Ultra Sensitive Rat Insulin ELISA Kit, 2nd Gen

The Rat Insulin ELISA (Ultra Sensitive), 2nd Gen is used to quantify levels of rat insulin in serum, plasma, cell culture, and fluid using only a 5 µL sample volume.
Highlights
  • Highly sensitive using only a 5 µL sample
  • Dynamic range of 0.1 - 13 ng/mL
  • Easy to use procedure with only one wash step and all liquid reagents
  • No cross-reactivity to rat proinsulin
  • No cross-reactivity to human insulin

For bulk purchases: Save money with our 10-kit bundle,  order online!

If you are using our kit in your publication, please reference it as Crystal Chem Cat# 62300, RRID:AB_3662575. Please let us know, and we'll thank you with a Amazon Gift card!

Product Specifications

Catalog # 62300
Sample Size 5 µL
Sample Types Serum, plasma, cell culture, or fluid
Tests 96 wells (12 x 8 well strips)
Reagents In liquid form (incl. standards)
Assay Range 0.1 - 13 ng/mL
Assay Time 3 hrs
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Regulatory Research Use Only
Distribution Worldwide
Assay and protocol summary Assay and protocol summary
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Additional Info

Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.

Note: For bulk purchases, save money with our 10-kit bundle.

Summary of Ultra Sensitive Rat Insulin ELISA Kit, 2nd Gen


Remove the Microplate (marked "MIC") from the pouch.
↓
Dispense 50 µL of Assay Diluent (marked "DIL") per well.
↓
Pipette 5 µL of the sample (or ready-to-use mouse insulin standard) per well.
↓
Dispense 50 µL of 1X antibody-HRP conjugate per well.
↓
Cover wells with plate seal, and then gently shake the plate to mix. Incubate the
microplate for 2.5 hours at room temp.
↓
Wash each well six times with 300uL wash buffer.
↓
Dispense 100 µL of Substrate Solution (marked "SUB") per well.
↓
Cover wells with plate seal and incubate the microplate for 30 mins at room temp
while avoiding exposure to light.
↓
Dispense 100 µL of Stop Solution (marked "STOP") per well.
↓
Measure absorbance within 15 mins at 450nm and subtract 630nm values.
↓
Calculate insulin concentrations using the standard curve.



Specificity:

Substance Reactivity*
Rat Insulin 100%
Rat Leptin Not Detected
Rat C-Peptide Not Detected
Rat Proinsulin Not Detected
Human Insulin Not Detected
* Not Detected indicates cross reactivity of less than 5%.



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