Rat TNF-a ELISA Kit (96-well)
Référence EA100164
Conditionnement : 1x96wells
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| Product Data | |
| ACCN | NM_012675 |
|---|---|
| Assay Length | 3 hour |
| Assay Platform | 96-well plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Type | Sandwich |
| Background | The prototype ligand of theTNFsuperfamily, TNF-./TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis .TNF-. is produced by activated macrophages and other cell types including T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells and some tumor cells . Rat TNF-. cDNA encodes a 235 amino acid (aa) residue type II membrane protein . The 156 aa residue soluble TNF-.is released from the C-terminus of the membrane-anchored TNF-. by TNF-.-converting enzyme (TACE), a matrix metalloprotease . The membrane-anchored form of TNF-. has been shown to have lytic activity and may also have an important role in intercellular communication . The biologically active TNF-. has been shown to exist as a trimer .TNF-. is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms . Deficiencies in either TNF-. or its receptors can increase susceptibility to infection by intracellular pathogens . TNF-. may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers . Other potential physiological roles for TNF-. and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells . |
| Components |
|
| Curve Range | 0.03 - 2.0 ng/mL |
| figure_legend | Representative standard curve for TNF-a ELISA.TNF-a was diluted in serial two-fold steps in Sample Diluent. |
| Format | 96-well strip plate |
| Gene Symbol | Tnf |
| Interference | No significant interference observed with available related molecules. |
| Locus ID | 24835 |
| Reactivity | Rat |
| Reactivity | Rat |
| Reactivity | Rat |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Sensitivity | 15pg/mL |
| Signal | Colorimetric |
| Species | Rat |
| Specificity | Natural and recombinant Rat TNF-a Ligand |
| Standard Curve | /assets/images/elisa/kits/128/cre0003-096-1-e.jpg |
| Target Symbol | Tnf |
| Description | Rat TNF-a ELISA Kit (96-well) |