Images
Result Data
Note

Result Data

Result Data

Detection of ROS in Hela cells. Hela cells were seeded overnight at 15000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated (1 mM H2O2 or 100 uM tert-butyl hydroperoxide) or untreated (control) for 30 min at 37°C. The ROS Deep Red stain solution (100 uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. The fluorescence signal was monitored at Ex/Em = 650/675 nm (cut off = 665 nm) with bottom read mode using FlexStation.

  • Specifications

    Product Description

    ROS Activity Assay Kit is a quantitative fluorometric determination of mitochondrial ROS activity.

    Suitable Sample

    Cell Culture

    Label

    Fluorometric

    Detection Method

    650/675 nm

    Assay Type

    Quantitative

    Regulation Status

    For research use only (RUO)

    Storage Instruction

    Store the kit at -20°C. For best results, please store the components as described in the protocol.

    Note

    Result Data

  • Applications

    Functional Study

    Flow Cytometry

  • Publication Reference