The RUNX2 FISH probe is designed to hybridize to the RUNX2 gene and is primarily used for detecting amplifications and deletions associated with the gene. This probe is FISH confirmed on normal peripheral blood metaphase spreads and interphase nuclei. The probe can be labeled in one of five colors. Each probe is sold in a 20 test kit (approximately 20 slides - 22x22 mm area) and includes hybridization buffer. Please note that due to design optimizations, are subject to change.

** This product is for in vitro and research use only. This product is not intended for diagnostic use.

Gene Summary

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]

Gene Details

Gene Symbol: RUNX2

Gene Name: Runt Related Transcription Factor 2

Chromosome: CHR6: 45296053-45518819

Locus: 6p21.1

References

Lineage-specific RUNX2 super-enhancer activates MYC and promotes the development of blastic plasmacytoid dendritic cell neoplasm

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a particularly aggressive acute leukemia. BPDCN patients display significantly higher levels of RUNX2 compared to other leukemia patients, due to the RUNX2 super-enhancers harbored by BPDCN cells. Because MYC translocations are also recurrent abnormalities in BPDCN, the team wanted to determine whether RUNX2 super-enhancers also upregulate MYC expression in these tumors. Empire Genomics' RUNX2 probe was used to detect RUNX2 amplification in a series of BPDCNs. Results demonstrated that RUNX2 super-enhancers are in fact hijacked to activate MYC via t(6,8) in BPDCN.

Product Details

Product: RUNX2 FISH Probe

Test Kits: 20 (40 μL)

ISH Buffer: 200 μL

SKU: RUNX2-20-OR

Material Safety Data Sheet: MSDS.pdf

Turnaround Time: 7-10 Business Days

Shipping Time: 1-2 Day Expedited Shipping