S100A8 ELISA Kit (Human)
Référence OKEH01279
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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| Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology S100A8 ELISA Kit (Human) (OKEH01279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100A8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100A8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100A8 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.2% (n = 20) Mean Inter-assay CV%: < 7.9% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.195 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Protein S100-A8 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | S100A8 |
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| Gene Full Name | S100 calcium binding protein A8 |
| Alias Symbols | 60B8AG;CAGA;calgranulin A;Calgranulin-A;calprotectin L1L subunit;CFAG;CGLA;CP-10;cystic fibrosis antigen;L1Ag;leukocyte L1 complex light chain;MA387;MIF;migration inhibitory factor-related protein 8;MRP8;NIF;P8;protein S100-A8;S100 calcium-binding protein A8;S100-A8;urinary stone protein band A. |
| NCBI Gene Id | 6279 |
| Protein Name | P05109 |
| Description of Target | Protein S100-A8 |
| Uniprot ID | https://www.uniprot.org/uniprot/P05109 |
| Protein Accession # | NP_002955.2 |
| Nucleotide Accession # | NM_002964.4 |
| Protein Size (# AA) | 93 |
| Molecular Weight | 11 kDa |