Abx941273-5nmol | siRNA Negative Control Clinisciences

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Datasheet SDS

siRNA Negative Control. Negative Control for experiments using targeted siRNA transfection. Recommended for evaluating RNAi off-target effects, and in order to verify the accuracy of gene-specific siRNA-dependent RNAi.

Reactivity

General (All species)

Tested Applications

RNAi

Host

Synthetic

Recommended dilutions

Optimal dilutions/concentrations should be determined by the end user.

Plate (wells)	Final Medium Volume (ml)	Final siRNA Concentration (nM)	20 µM siRNA Volume (µl)	Lipofectamine 2000 Volume (µl)
96	0.1	100	0.5	0.25
		50	0.25	0.25
		10	0.05	0.25
24	0.5	100	2.5	1
		50	1.25	1
		10	0.25	1
12	1	100	5	2
		50	2.5	2
		10	0.5	2
6	2	100	10	5
		50	5	5
		10	1	5

Form

Lyophilized

Purity

> 97%

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Storage

Shipped at 4 °C. Store at -20 °C for up to one year.

Specificity

siRNA Negative Control is a non-targeting 21 nt siRNA.

Availability

Shipped within 5-15 working days.

Note

This product is for research use only.

Directions for use

Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube.
Resuspend the siRNA oligos to an appropriate concentration with DEPC water (e.g. resuspend one vial of 5 nmol siRNA oligo in 250 µl of DEPC water for a final concentration of 20 µM).
Transfect with 10 nM - 100 nM siRNA 48 to 72 hours prior to cell lysis.

Research Articles on siRNA Negative Control

siRNA Negative Control

Référence abx941273-5nmol

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