SSH3BP1 (ABI1) Mouse Monoclonal Antibody [Clone ID: 1B9]
Specifications
Product Data | |
Clone Name | 1B9 |
Applications | IHC, IP, WB |
Recommended Dilution | Western blot: 1 μg / ml for chemiluminescence detection system. Immunoprecipitation: 2 μg / 200 μL of cell extract from 5x106 cells. Immunohistochemistry on paraffin sections: 5 μg / ml. Heat treatment is necessary for paraffin embedded sections. Microwave oven; 2 times for 10 minutes each in citrate buffer (pH 6.5). For details see protocol below. This antibody was used in Immunocytochemistry in ref. 1. |
Reactivities | Hamster, Human, Mouse |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human recombinant Abi-1 expressed in E. coli |
Specificity | This antibody reacts with Abi-1. |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliqouts) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Predicted Protein Size | 65 kDa |
Gene Name | abl interactor 1 |
Database Link | |
Background | Abl interactor (Abi) proteins are c-Abl-binding proteins that bind to both the Src homology 3 (SH3) domain and the C-terminal proline-rich regions of Abl kinase through dual SH3-PXXP interactions. Two highly related genes, abi-1 and abi-2, were cloned. In addition to the interaction with Abl kinase, Abi proteins also interact with other signaling molecules. Recently, it has been reported that Abi-1 dramatically promoted c-Abl-mediated tyrosine (Tyr296) phosphorylation of Mena [mammalian homologue of Drosophila Enabled (Ena)] by interacting with both proteins. |
Synonyms | Abl interactor 1, Abelson interactor 1, Abi-1, e3B1, Spectrin binding protein, Nap1-binding protein, Abl-binding protein 4, AblBP4 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunoprecipitation Immunohistochemical staining for paraffin-embedded sections: SAB method |
Reference Data |
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