A8005-100mg | Sulfo-NHS-SS-Biotin [325143-98-4] Clinisciences

Sulfo-NHS-SS-biotin(sulfosuccinimidyl-20(biotinamido)ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent. The presence of the negatively charged sulfonate group in the chemical structure of sulfo-NHS-SS-biotin makes it a water-soluble biotinylation reagent that can be directly added to aqueous reactions without prior dissolution of organic solvents. Although no prior dissolution is required, an aqueous stock solution of sulfo-NHS-SS-biotin must be prepared rapidly and used immediately in case of the occurrence of hydrolysis of the active ester. Sulfo-NHS-SS-biotin has been used to react with amine-containing proteins and other molecules forming a complex which further interacts with avidin or streptavidin probes and to purify targeted molecules using affinity chromatography on a column of immobilized avidin or streptavidin.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

1. Guoli Song, et al. "SELENOK-dependent CD36 palmitoylation regulates microglial functions and Aβ phagocytosis." Redox Biol. 2024 Apr:70:103064. PMID: 38320455
2. Weixiang Bian, Hua Jiang, et al. "A spatially defined human Notch receptor interaction network reveals Notch intracellular storage and Ataxin-2-mediated fast recycling." Cell Rep. 2023 Jul 25;42(7):112819. PMID: 37454291
3. Jian Chen, Shizhen Qiu, et al. "Tuning charge density of chimeric antigen receptor optimizes tonic signaling and CAR-T cell fitness." Cell Res. 2023 May;33(5):341-354. PMID: 36882513
4. Asma B Salek, Emily T Claeboe, et al. "Spinophilin dependent regulation of GluN2B‐containing NMDAR‐dependent calcium influx, GluN2B surface expression, and cleaved caspase expression." Synapse 2023 Feb 04; PMID: 36738175
5. Jonathan Perr, Andreas Langen, et al. "RNA binding proteins and glycoRNAs form domains on the cell surface for cell penetrating peptide entry." bioRxiv. September 05, 2023.
6. Neuza Domingues, Steve Catarino, et al. "Cx43 promotes exocytosis of damaged lysosomes through actin remodelling." Research Square. December 21st, 2022.
7. Shaoqin Zheng, Jiahui Lin, et al. "Aberrant Cholesterol Metabolism and Wnt/β‐Catenin Signaling Coalesce via Frizzled5 in Supporting Cancer Growth." Adv Sci (Weinh). 2022 Oct;9(28):e2200750. PMID: 35975457
8. Angela L Whittsette, Ya-Juan Wang, et al. "The Endoplasmic Reticulum Membrane Complex Promotes Trafficking And Function Of GABAA Receptors." iScience. 2022 Jul 13;25(8):104754. PMID: 35938049
9. Meng Wang, Edmund Cotter, et al. "Pharmacological activation of ATF6 remodels the proteostasis network to rescue pathogenic GABAA receptors." Cell Biosci. 2022 Apr 27;12(1):48. PMID: 35477478
10. Zheng Niu, YiLing Zhang, et al. "Decreased NHE3 activity in intestinal epithelial cells in TGEV and PEDV-induced piglet diarrhea." Vet Microbiol. 2021 Dec;263:109263. PMID: 34749283
11. Jobe L. Ritchie, Jennifer L. Walters, et al. "Basolateral amygdala corticotropin-releasing factor receptor type 1 regulates context-cocaine memory strength during reconsolidation in a sex-dependent manner." Neuropharmacology. 2021 Dec 1;200:108819. PMID: 34610289
12. Zhenhui Song, Tao Yan, et al. "Reduced activity of intestinal surface Na+/H+ exchanger NHE3 is a key factor for induction of diarrhea after PEDV infection in neonatal piglets." Virology. 2021 Nov;563:64-73. PMID: 34464882
13. Yang Y, Yu Q, et al. "Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway." Virus Res. 2020;280:197901. PMID: 32070687
14. Saladi S, Boos F, et al. "The NADH Dehydrogenase Nde1 Executes Cell Death after Integrating Signals from Metabolism and Proteostasis on the Mitochondrial Surface." Mol Cell. 2019 Oct 18. pii: S1097-2765(19)30732-4. PMID: 31668496
8. Sheridan J.S. Carrington. "Differential Glycosylation of the Inwardly Rectifying Potassium Channel Kir7.1 by G protein-coupled Receptors." Vanderbilt University. 2019.
16. Carrington S, Hernandez C, et al. "G protein-coupled receptors differentially regulate glycosylation and activity of the inwardly rectifying potassium channel Kir7.1." J Biol Chem. 2018 Sep 26. pii: jbc.RA118.003238. PMID: 30257863
17. Brasher MI, Martynowicz DM, et al. "Interaction of Munc18c and Syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumour cells." J Biol Chem. 2017 Aug 10. pii: jbc.M117.807438. PMID: 28798239

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• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the N-terminal-amine
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length—spacer arm is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain

Storage	Store at -20°C The product is not stable in solution, please dissolve it immediately before use.
M.Wt	606.7
Cas No.	325143-98-4
Formula	C₁₉H₂₇N₄NaO₉S₄
Synonyms	Biotin disulfide N-hydroxysulfosuccinimide ester
Solubility	≥30.33 mg/mL in DMSO; <2.68 mg/mL in EtOH; <2.74 mg/mL in H2O
Chemical Name	sodium;1-[3-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethyldisulfanyl]propanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
SDF	Download SDF
Canonical SMILES	C1C(C(=O)N(C1=O)OC(=O)CCSSCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Biotinylation method [1]:
Sample	468/EV cells and 468/uPAR cells.
Preparation method	Soluble in water, DMSO or DMF.
Reaction Conditions	1 mg/ml, 15minutes on ice
Applications	Cells in monolayer culture (1.5 × 106) were washed three times with ice-cold PBS and then treated with sulfo-NHS-SS-biotin (1mg/mL) for 15 minutes on ice. Biotinylation reactions were terminated with 100 mmol/L glycine in PBS. After washing with PBS, cell extracts were prepared in radioimmunoprecipitation assay (RIPA) buffer (20 mmol/L sodium phosphate, 150 mmol/L NaCl (pH 7.4), 1% NP40, 0.1% SDS, and 0.5% deoxycholic acid) with protease inhibitor cocktail. Biotinylated membrane proteins were precipitated with streptavidin-sepharose. Proteins were eluted with SDS sample buffer, resolved by SDS-PAGE, electrotransferred to polyvinylidene difluoride (PVDF) membranes, and probed with primary antibodies.
References: [1]. Minji Jo, Boryana M. Eastman, Drue L. Webb, et al. Cell Signaling by Urokinase-type Plasminogen Activator Receptor Induces Stem Cell-like Properties in Breast Cancer cells . Cancer Res, 2010;70:8948-8958

Description	Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent
Targets
IC50

Purity = 98.00%

Sulfo-NHS-SS-Biotin [325143-98-4]

Référence A8005-100mg

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