TCEP (hydrochloride) [51805-45-9]
Référence HY-W011500-1ml
Conditionnement : 10mM/1mL
Marque : MedChemExpress
TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a non-thiol reducing agent that is more stable and produces a faster S-S reductive reaction than other chemical reductants. TCEP hydrochloride is a trialkylphosphine, selectively reduces protein disuldes without altering the properties or interacting with thiol-directed agents in the reaction mixture. TCEP hydrochloride is also a commonly used reducing agent in the DNA/AuNP chemistry.
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TCEP hydrochloride Chemical Structure
CAS No. : 51805-45-9
This product is a controlled substance and not for sale in your territory.
Based on 4 publication(s) in Google Scholar
Other Forms of TCEP hydrochloride:
- TCEP-d16 hydrochloride In-stock
Description |
TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a non-thiol reducing agent that is more stable and produces a faster S-S reductive reaction than other chemical reductants. TCEP hydrochloride is a trialkylphosphine, selectively reduces protein disuldes without altering the properties or interacting with thiol-directed agents in the reaction mixture. TCEP hydrochloride is also a commonly used reducing agent in the DNA/AuNP chemistry[1][2][3][4]. |
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In Vitro |
TCEP hydrochloride has been introduced which oers the prospect of serving as an alternative to the more commonly employed DTT in the NF-κB-DNA binding reactions in vitro, using recombinant p50 protein and a 32P-labelled κB oligonucleotide. DTT promotes NF-κB-DNA binding in concentrations from 0.25 to 2.6 mM in binding reactions. However, in the presence of 0.25 mM DTT, inhibition of NF-κB binding is seen only at Hg2+ concentrations greater than 100 μM and results are highly variable. In contrast, TCEP hydrochloride promotes NF-κB-DNA binding in a dose-related manner in concentrations from 0.25 to 6 mM. In the presence of even 6 mM TCEP hydrochloride, Hg2+ prevents NF-κB-DNA binding at concentrations as low as 20 μM in binding reactions[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
286.65 |
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Formule |
C9H16ClO6P |
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CAS No. |
51805-45-9 |
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Appearance |
Solid |
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Color |
White to light yellow |
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SMILES |
O=C(O)CCP(CCC(O)=O)CCC(O)=O.Cl |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
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Solvant et solubilité |
In Vitro:
DMSO : 100 mg/mL (348.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 50 mg/mL (174.43 mM; ultrasonic and adjust pH to 7 with NaOH) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Pureté et documentation | |||||||||||||
Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 3.4886 mL | 17.4429 mL | 34.8857 mL | 87.2144 mL |
5 mM | 0.6977 mL | 3.4886 mL | 6.9771 mL | 17.4429 mL | |
10 mM | 0.3489 mL | 1.7443 mL | 3.4886 mL | 8.7214 mL | |
15 mM | 0.2326 mL | 1.1629 mL | 2.3257 mL | 5.8143 mL | |
20 mM | 0.1744 mL | 0.8721 mL | 1.7443 mL | 4.3607 mL | |
25 mM | 0.1395 mL | 0.6977 mL | 1.3954 mL | 3.4886 mL | |
30 mM | 0.1163 mL | 0.5814 mL | 1.1629 mL | 2.9071 mL | |
40 mM | 0.0872 mL | 0.4361 mL | 0.8721 mL | 2.1804 mL | |
50 mM | 0.0698 mL | 0.3489 mL | 0.6977 mL | 1.7443 mL | |
60 mM | 0.0581 mL | 0.2907 mL | 0.5814 mL | 1.4536 mL | |
80 mM | 0.0436 mL | 0.2180 mL | 0.4361 mL | 1.0902 mL | |
100 mM | 0.0349 mL | 0.1744 mL | 0.3489 mL | 0.8721 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
TCEP hydrochloride Related Classifications
- Others
- Others