Tubulin, alpha (BSA & Azide Free) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) (APC)

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.||Applications:|Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.||Recommended Dilutions:|Western Blot: 1-2ug/ml for 2hrs at RT|Immunohistochemistry (Formalin/paraffin): 1:400 for 20 minutes at RT. No special pretreatment is requiredfor stainingof formalin-fixed, paraffin-embedded tissues.|Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G.|Optimal dilutions to be determined by the researcher.||Positive Controls: |LS174T, MAD109 cells, skin and lung||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.