Specifications

Product Data
Applications	ELISA, IF, IHC
Recommended Dilution	ELISA Immunofluorescence: Direct FA staining of target antigens in a permissive tissue culture system. A starting range of 1/10-1/50 is suggested. Immunohistochemistry (Proteinase K digestion is recommended with formalin-fixed paraffin-embedded sections).
Reactivities	Vaccinia virus
Host	Rabbit
Clonality	Polyclonal
Immunogen	Lister Strain (mixture of virions and infected cell polypeptides).
Specificity	Recognizes purified virions. Does not cross-react with Parainfluenza (1-3), RSV, adenovirus, Influenza A & B or HSV-1. Does not react with uninfected cells. Reactive with Lister, Wyeth, New York City and MVA strains of Vaccinia.
Formulation	0.01M PBS, pH 7.2 Label: FITC State: Liquid purified IgG fraction Stabilizer: 10 mg/ml BSA Preservative: 0.09% Sodium Azide Label: Covalently coupled with high purity Isomer I of Fluorescein Isothiocyanate Care is taken to ensure complete removal of any free fluorescein from the final product
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	FITC
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Background	Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As Vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During Vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize Vaccinia virus infection and enable identification other viral and cellular proteins which participate in the Vaccinia virus entry process.
Reference Data