Vimentin (FLJ36605, VIM) (FITC)

Référence V2122-03C-250ul

Conditionnement : 250ul

Marque : US Biological

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V2122-03C Vimentin (FLJ36605, VIM) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
bovine
Swiss Prot
P48616
Isotype
IgG2a
Grade
Affinity Purified
Applications
FLISA IF IHC WB
Crossreactivity
Bo Ca Ch Hu Mk Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C

The binding region of this antibody has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alphahelical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa353 in murine vimentin (that could explain for the weak crossreaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).||Applications: |Suitable for use in Western Blot, FLISA, Immunofluorescence Microscopy and Immunohistochemistry. Other applications not tested. ||Recommended Dilution: |Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT; extended with paraffin. Protease pretreatment is required prior to antibody application.|Optimal dilution determined by the researcher. ||Positive Controls:|Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 5F310|Host: mouse|Source: bovine|Concentration: Not Determined |Form: Supplied as a liquid, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Vimentin (purified from bovine lens).|Specificity: Recognizes the intermediate filament protein of bovine vimentin at 57kD, which is present in all cells of mesenchymal origin. Species Crossreactivity: human, monkey, canine, chicken, amphibia (e.g. Xenopus laevis). Weaker mouse cross-reaction. Reactivities on Cultured Cells: RD Cells, glioma cells, fibroblasts (SV-80), MDCK.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Vimentin (purified from bovine lens).
Form
Supplied as a liquid, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the intermediate filament protein of bovine vimentin at 57kD, which is present in all cells of mesenchymal origin. Species Crossreactivity: human, monkey, canine, chicken, amphibia (e.g. Xenopus laevis). Weaker mouse cross-reaction. Reactivities on Cultured Cells: RD Cells, glioma cells, fibroblasts (SV-80), MDCK.
References
1. Heid HW, et al., Differentiation 37, 137-157, 1988. 2. Hermann H, et al., Differentiation 36, 234-254 (1987 Kasper M, et al., Histochemistry 93, 93-103, 1989. 3. Kasper M, et al., Differentiation 40, 207-214, 1989. 4. Moll I et al., Arch Dermatol Res 283, 300-309, 1991. 5. Gmi H, et al., Neuron, 14, 29-41, 1995. 6. Demirkesen C, et al., J Cutan Pathol 22: 518-535, 1995. 7. Hermann H, et al., J Mol Biol 234: 99-113, 1993. 8. Rogers KR, et al., Eur J Cell Biol 66: 136-150, 1995. 9. Bohn W, et al., Exp Cell Res 201: 1-7, 1992. 10. Hermann H, et al., J Mol Biol 223: 637-650, 1992. 11. Koeser J, et al., Exp Cell Res 285, 114-130, 2003 .