8OHDG ELISA Kit
Référence OKEH02553
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | ELISA-Comp | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology 8OHDG ELISA Kit (OKEH02553) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-8OHDG antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 8OHDG and incubated. The 8OHDG found in the sample or standards competes with the biotinylated 8OHDG for limited binding sites on the immobilized anti-8OHDG antibody. Excess unbound biotinylated 8OHDG and sample or standard 8OHDG is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 8OHDG captured in the well and inversely proportional to the amount of 8OHDG which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 85% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.3% (n = 20) Mean Inter-assay CV%: < 7.2% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: 8OHDG Target Name: 8-Oxo-2'-deoxyguanosine | ||||||||||||||||||||||
| :: | Pubchem: 73318 | ||||||||||||||||||||||
| :: | Chemical formula: C10H13N5O5 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.27 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General 8-Oxo-2'-deoxyguanosine | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | 8OHDG, 8-Oxo-2'-deoxyguanosine, 8OHDG, 8-Hydroxydeoxyguanosine |
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| Protein Name | 8OHDG |
| Description of Target | 8-Oxo-2'-deoxyguanosine (8-oxo-dG) is an oxidized derivative of deoxyguanosine. 8-oxo-dG is one of the major products of DNA oxidation. Concentrations of 8-oxo-dG within a cell are a measurement of oxidative stress. |