Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

ACE2/CHO-K1 Stable Cell Line

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Référence 14-523ACL

Conditionnement : 1vial

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Téléphone : +1 850 650 7790

Marque : Abeomics

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

ACE2/CHO-K1 Stable Cell Line

Fig-1: Detection of human ACE2 in the ACE2/CHO-K1 stable cell line by Flow Cytometry [Cell surface staining] using anti-human ACE2 antibody (Clone AC18F; Abeomics Cat. No.: 10-10031-AT488). Parental CHO-K1 cells (Green); ACE2/CHO-K1 cells (Red).

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Fig-1: Detection of human ACE2 in the ACE2/CHO-K1 stable cell line by Flow Cytometry [Cell surface staining] using anti-human ACE2 antibody (Clone AC18F; Abeomics Cat. No.: 10-10031-AT488). Parental CHO-K1 cells (Green); ACE2/CHO-K1 cells (Red).

Fig-2: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/CHO-K1 stable cell line. ACE2/CHO-K1 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein (Abeomics, Cat. No. 21-1005-B) and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.

Fig-3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/CHO-K1 stable cell line. ACE2/CHO-K1 cells were incubated with various concentrations of biotinylated SARS-Cov-2 Spike RBD protein (Abeomics, Cat. No. 21-1005-B) and analyzed through In-Cell ELISA. RBD binds to ACE2/CHO-K1 with an EC50 of 1.33 µg/ml.

Fig-4: Neutralization of binding between ACE2/CHO-K1 cells and SARS-Cov-2 Spike RBD protein by the Recombinant Anti-SARS-CoV-2 Spike RBD antibodies (CR3022: Abeomics, Cat. No. 10-2004 and ABMX-002: Abeomics, Cat. No. 10-2005). CHO-K1/ACE2 stable cells were incubated with various concentrations of RBD antibodies in the presence of biotinylated SARS-Cov-2 Spike RBD protein (Abeomics, Cat. No. 21-1005-B) and analyzed through In-Cell ELISA using HRP-Streptavidin for detection.

Product code: 14-523ACL

Application :	Functional Assay

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