Acridine Orange Hydrochloride Hydrate (CI 46005)

Acridine Orange Hydrochloride is the hydrocloride salt of Acridine Orange Stain, which is a fluorescent nucleic acid binding dye that interacts with both DNA and RNA. When bound to DNA, it emits green fluorescence (Em=525 nm) and when bound to RNA, it emits red fluorescence (Em=~650 nm). Acridine Orange Stain has significantly distinguished cell death by apoptosis and necroptosis in human oligodendrocytes and mouse models.||Synonyms:|3,6-Bis(dimethylamino)acridine hydrochloride; CI 46005||CAS No:|65-61-2||Molecular Formula:|C17H19N3·HCl·xH2O||Molecular Weight:|301.82||Appearance:|Dark orange or brick red powder||Purity:|≥99% (HPLC) ||Melting Point:|284-287°C (lit.)||UV Ratios (10mg/L in water):|489-495 nm = 1.160|265-271 nm = 1.160|227-233 nm = 0.464||Extinction Co-Efficient (water):|E (489-495 nm) : ≥35000 |E (265-271 nm) : ≥35000 |E (227-233 nm) : ≥14000||Water (KF):|≤20%||Solubility:|Water: 0.1%, clear, orange to red solution||Storage and Stability:|May be stored at RT. Light sensitive.