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> Animal PDGFBB ELISA Kit

Animal PDGFBB ELISA Kit

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Référence orb866943-96

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    Animal PDGFBB ELISA Kit

    Catalog Number: orb866943

    Catalog Numberorb866943
    CategoryAssays and Kits
    DescriptionSandwich Animal PDGFBB ELISA Kit
    Tested applicationsELISA
    ReactivityAnimal
    Range1.57-100 ng/mL
    Concentration2000 pg/mL
    Sensitivity0.59 ng/mL
    Protein Length3.5h
    Alternative namesPDGF-BB ELISA Kit
    NoteFor research use only
    Application notesStandard: 100 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse PDGFBB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse PDGFBB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse PDGFBB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse PDGFBB in the samples is then determined by comparing the OD of the samples to the standard curve
    Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Expiration DatePlease enquire.
    Animal PDGFBB ELISA Kit