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> Anti-ACAA2 antibody (1-250)

Anti-ACAA2 antibody (1-250)

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Référence NB-22-49326-200

Conditionnement : 200uL(2x100uL)

Marque : Neo Biotech

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Téléphone : +1 850 650 7790

  • Immunofluorescence analysis of U-2 OS cells using ACAA2 Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ACAA2 Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ACAA2 Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ACAA2 in paraffin-embedded rat liver tissue using ACAA2 Rabbit polyclonal antibody at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ACAA2 in paraffin-embedded human liver tissue using ACAA2 Rabbit polyclonal antibody at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using ACAA2 Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

General Info

Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACAA2 (1-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: ACAA2
Gene ID: 10449
Uniprot ID: THIM_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACAA2 (NP_006102.2).
Immunogen Sequence: MALLRGVFVVAAKRTPFGAY GGLLKDFTATDLSEFAAKAA LSAGKVSPETVDSVIMGNVL QSSSDAIYLARHVGLRVGIP KETPALTINRLCGSGFQSIV NGCQEICVKEAEVVLCGGTE SMSQAPYCVRNVRFGTKLGS DIKLEDSLWVSLTDQHVQLP MAMTAENLAVKHKISREECD KYALQSQQRWKAANDAGYFN DEMAPIEVKTKKGKQTMQVD EHARPQTTLEQLQKLPPVF

Description

Function In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
Protein Name 3-Ketoacyl-Coa Thiolase - Mitochondrial
Acetyl-Coa Acetyltransferase
Acetyl-Coa Acyltransferase
Acyl-Coa Hydrolase - Mitochondrial
Beta-Ketothiolase
Mitochondrial 3-Oxoacyl-Coa Thiolase
T1
Database Links Reactome: R-HSA-77289
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-3-Ketoacyl-Coa Thiolase - Mitochondrial antibody
Anti-Acetyl-Coa Acetyltransferase antibody
Anti-Acetyl-Coa Acyltransferase antibody
Anti-Acyl-Coa Hydrolase - Mitochondrial antibody
Anti-Beta-Ketothiolase antibody
Anti-Mitochondrial 3-Oxoacyl-Coa Thiolase antibody
Anti-T1 antibody
Anti-ACAA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance