Anti-ADGRG1 antibody (Extracellular Loop) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55-0.65 µg/µl
Dilution Range:	WB: 1:500-1:750 DB: 1:20, 000-1:50, 000 ELISA: 1:20, 000-1:50, 000 IP: 1:200 IHC: 1:100
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	ADGRG1
Gene ID:	9289
Uniprot ID:	AGRG1_HUMAN
Immunogen Region:	Extracellular Loop
Immunogen:	Synthetic peptide taken from large extracellular loop of the human GPCR56 protein. Peptide was conjugated to Keyhole Limpet Haemocyanin (KLH).

Tissue Specificity	Widely distributed with highest levels found in thyroid gland, brain and heart. Expressed in a great number of tumor cells. Expression is down-regulated in different tumors from highly metastatic cells.
Post Translational Modifications	Autoproteolytically cleaved into 2 fragments.the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1 CT) predominantly remain associated and non-covalently linked. Shedding to yield the secreted ADGRG1 N-terminal fragment seems to involve metalloprotease(s). N-glycosylated. Contains sialic acid residues. Ubiquitinated. Undergoes polyubiquitination upon activation.
Function	Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination. Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12. Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche. Plays a critical role in cancer progression by inhibiting VEGFA production threreby inhibiting angiogenesis through a signaling pathway mediated by PRKCA. Plays an essential role in testis development. ADGRG1 N-terminal fragment: Plays a critical role in cancer progression by activating VEGFA production and angiogenesis through a signaling pathway mediated by PRKCA.
Protein Name	Adhesion G-Protein Coupled Receptor G1 G-Protein Coupled Receptor 56 Protein Tm7xn1 Cleaved Into - Adgrg1 N-Terminal Fragment Adgrg1 Nt Gpr56 N-Terminal Fragment Gpr56 Nt Gpr56(N Gpr56 Extracellular Subunit Gpr56 Subunit Alpha - Adgrg1 C-Terminal Fragment Adgrg1 Ct Gpr56 C-Terminal Fragment Gpr56 Ct Gpr56(C Gpr56 Seven-Transmembrane Subunit Gpr56 7tm Gpr56 Subunit Beta
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein Adgrg1 N-Terminal Fragment: Secreted Adgrg1 C-Terminal Fragment: Membrane Raft Interaction With Its Ligand Col3a1 Leads To The Release Of Adgrg1 Nt From The Membrane And Triggers The Association Of Adgrg1 Ct With Lipid Rafts
Alternative Antibody Names	Anti-Adhesion G-Protein Coupled Receptor G1 antibody Anti-G-Protein Coupled Receptor 56 antibody Anti-Protein Tm7xn1 Cleaved Into - Adgrg1 N-Terminal Fragment antibody Anti-Adgrg1 Nt antibody Anti-Gpr56 N-Terminal Fragment antibody Anti-Gpr56 Nt antibody Anti-Gpr56(N antibody Anti-Gpr56 Extracellular Subunit antibody Anti-Gpr56 Subunit Alpha - Adgrg1 C-Terminal Fragment antibody Anti-Adgrg1 Ct antibody Anti-Gpr56 C-Terminal Fragment antibody Anti-Gpr56 Ct antibody Anti-Gpr56(C antibody Anti-Gpr56 Seven-Transmembrane Subunit antibody Anti-Gpr56 7tm antibody Anti-Gpr56 Subunit Beta antibody Anti-ADGRG1 antibody Anti-GPR56 antibody Anti-TM7LN4 antibody Anti-TM7XN1 antibody Anti-UNQ540 antibody Anti-PRO1083 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance