Detection of human IgG4 in monkey serum. Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.

Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.

Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.

A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG4 rabbit monoclonal antibody, Clone RM120.

ELISA showing RM120 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM120 and a monkey IgG binding antibody (positive control) was used as the detection antibody.

Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG4 antibody, Clone RM120, at a 1:50,000 dilution.

Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA