| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Escherichia coli |
| Reactivity | E. coli |
| Clonality | Polyclonal |
Application 
| WB, E, I, LCI |
| Application Note | Anti-LMW Host Cell Proteins Antibody detects over 270 E.coli LMW proteins and has been tested by 2D-Page, western blotting, and ELISA. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-LMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli (BL21). |
| Preservative | 0.01% (w/v) Sodium Azide |
| Purity | LMW Host Cell Protein A purified antibody is directed against low molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography. |
|---|---|
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@clinisciences.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli. This antibody has been analyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
