Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins)
Référence 610-103-121
Conditionnement : 1mg
Marque : Rockland Immunochemicals
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Téléphone : +1 850 650 7790
Specifications for Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed
Product Details
Goat Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) - 610-103-121
Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
Goat
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Mouse
Mouse IgG whole molecule
HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Application Details
ELISA, WB
IHC, Other - View References
Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and western blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Anti-Mouse IgG Peroxidase Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
References (28)
Wang W et al. (2023). Matrix stiffness regulates tumor cell intravasation through expression and ESRP1-mediated alternative splicing of MENA. Cell Rep.
Applications
WB, IB, PCA PubMed
Schenke M et al. (2021). Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1. Toxins (Basel).
Applications
WB, IB, PCA PubMed
Yingling CV et al. (2021). FHOD formin and SRF promote post-embryonic striated muscle growth through separate pathways in C. elegans. Exp Cell Res.
Applications
WB, IB, PCA PubMed
Schottelndreier D et al. (2020). Low Density Lipoprotein Receptor-Related Protein-1 (LRP1) Is Involved in the Uptake of Clostridioides difficile Toxin A and Serves as an Internalizing Receptor. Front Cell Infect Microbiol.
Applications
WB, IB, PCA PubMed
Nishida T et al. (2020). Suppression of adipocyte differentiation by low‐intensity pulsed ultrasound via inhibition of insulin signaling and promotion of CCN family protein 2. J Cell Biochem.
Applications
WB, IB, PCA PubMed
Timon-Gomez A et al. (2020). Distinct roles of mitochondrial HIGD1A and HIGD2A in respiratory complex and supercomplex biogenesis. Cell Rep.
Applications
WB, IB, PCA PubMed
Kim MR et al. (2020). TET2 directs mammary luminal cell differentiation and endocrine response. Nat Commun.
Applications
WB, IB, PCA PubMed
Timon-Gomez A et al. (2020). Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis. STAR Protoc.
Applications
WB, IB, PCA PubMed
Yang X, He Z, Zhang G, et al. (2020). Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 of Brucella spp. Front Cell Infect Microbiol.
Applications
IHC, ICC, Histology PubMed
Amanat F, Stadlbauer D, Strohmeier S, et al. (2020). A serological assay to detect SARS-CoV-2 seroconversion in humans. Nat. Med.
Applications
NEU PubMed
Genth et al. (2019). Difference in Mono-O-Glucosylation of Ras Subtype GTPases Between Toxin A and Toxin B From Clostridioides difficile Strain 10463 and Lethal Toxin From Clostridium sordellii Strain 6018. Frontiers in Microbiology
Applications
WB, IB, PCA PubMed
Brown et al. (2018). FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. Oncotarget
Applications
WB, IB, PCA PubMed
Fan et al. (2018). Upregulation of miR‑185 promotes apoptosis of the human gastric cancer cell line MGC803. Molecular Medicine Reports
Applications
WB, IB, PCA PubMed
Schöttelndreier et al. (2018). Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B. Frontiers in Microbiology
Applications
WB, IB, PCA PubMed
Lobo-Jarne T, Nývltová E, Pérez-Pérez R, et al. (2018). Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep.
Applications
WB, IB, PCA PubMed
Ercan et al. (2017). Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex. Journal of Experimental Medicine
Applications
WB, IB, PCA PubMed
Shen HY et al. (2017). Antitumor activity of fucoidan in anaplastic thyroid cancer via apoptosis and anti-angiogenesis. Mol Med Rep.
Applications
WB, IB, PCA PubMed
Langer N et al. (2017). Determination of cross-reactivity of poly-and monoclonal antibodies for synthetic cannabinoids by direct SPR and ELISA. Forensic Sci Int.
Applications
E, EIA PubMed
Ciesielska A et al. (2017). Bis (monoacylglycero) phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol.
Applications
EIA, ELISA PubMed
Lampi et al. (2016). Simvastatin Ameliorates Matrix Stiffness-Mediated Endothelial Monolayer Disruption. PLOS One
Applications
WB, IB, PCA PubMed
Schelle et al. (2016). Role of p38alpha/beta MAP Kinase in Cell Susceptibility to Clostridium sordellii Lethal Toxin and Clostridium difficile Toxin B. Toxins
Applications
WB, IB, PCA PubMed
Dickerman et al. (2015). The protein activator of protein kinase R, PACT/RAX, negatively regulates protein kinase R during mouse anterior pituitary development. The FEBS Journal
Applications
WB, IB, PCA PubMed
Horn P et al. (2012). Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc.
Applications
WB, IB, PCA PubMed
LeVine H et al. (2012). Dihydroxybenzoic acid isomers differentially dissociate soluble biotinyl-Aβ(1-42) oligomers. Biochemistry
Applications
E, EIA PubMed
Zhang X et al. (2011). Vaccination with different M2e epitope densities confers partial protection against H5N1 influenza A virus challenge in chickens. Intervirology.
Applications
WB, IB, PCA PubMed
McCall LI et al. (2010). Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. Mol Microbiol.
Applications
WB, IB, PCA PubMed
Reeves TM et al. (2010). Proteolysis of submembrane cytoskeletal proteins ankyrin-G and αII-spectrin following diffuse brain injury: a role in white matter vulnerability at Nodes of Ranvier. Brain Pathology (Zurich, Switzerland)
Applications
WB, IB, PCA PubMed
Van Komen JS et al. (2005). The polybasic juxtamembrane region of Sso1p is required for SNARE function in vivo. Eukaryot Cell.
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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