Anti-MUC2 antibody (850-5289)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:100-1:500 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MUC2
Gene ID:	4583
Uniprot ID:	MUC2_HUMAN
Immunogen Region:	850-5289
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 850-5289 of human MUC2 (NP_002448.4).
Immunogen Sequence:	VCTQAVCHGTCSIYGSGHYI TFDGKYYDFDGHCSYVAVQD YCGQNSSLGSFSIITENVPC GTTGVTCSKAIKIFMGRTEL KLEDKHRVVIQRDEGHHVAY TTREVGQYLVVESSTGIIVI WDKRTTVFIKLAPSYKGTVC GLCGNFDHRSNNDFTTRDHM VVSSELDFGNSWKEAPTCPD VSTNPEPCSLNPHRRSWAEK QCSILKSSVFSICHSKVDPK PFYEACVHDSCSCDTGGDC

Tissue Specificity	Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Post Translational Modifications	O-glycosylated. O-glycosylation is required for mucin assembly. Goblet cells synthesize two forms of mucin that differ in branched chain O-glycosylation and the site of production in the colon. May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
Function	Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine. MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange. Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells. Binds both Cu(2+) and its reduced form, Cu(1+), at two juxtaposed binding sites: Cu(2+), once reduced to Cu(1+) by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site. MUC2-bound Cu(1+) is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells. Mucin gels store antimicrobial molecules that participate in innate immunity. Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body. Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon: a (1) 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon. 'Thick' mucus transits along the descending colon and is lubricated by a (2) 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus.
Protein Name	Mucin-2 Muc-2 Intestinal Mucin-2
Database Links	Reactome: R-HSA-5083625 Reactome: R-HSA-5083632 Reactome: R-HSA-5083636 Reactome: R-HSA-5621480 Reactome: R-HSA-913709 Reactome: R-HSA-977068
Cellular Localisation	Secreted In The Intestine Secreted Into The Inner And Outer Mucus Layers Before Secretion Mucin Polymers Are Stored In Dedicated Secretory Vesicles
Alternative Antibody Names	Anti-Mucin-2 antibody Anti-Muc-2 antibody Anti-Intestinal Mucin-2 antibody Anti-MUC2 antibody Anti-SMUC antibody

Information sourced from Uniprot.org

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