Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Anti-SARS-CoV-2 Spike Protein (S2) (MOUSE) Antibody

Imprimer

Référence 200-301-MU3

Conditionnement : 100ug

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Marque : Rockland Immunochemicals

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Téléphone : +1 850 650 7790

Specifications for SARS-CoV-2 Spike Protein (S2) Antibody

Product Details

Description: Anti-SARS-CoV-2 Spike Protein (S2) (MOUSE) Antibody - 200-301-MU3

Synonyms: mouse anti-SARS CoV 2 Spike S2 Protein Antibody, Spike glycoprotein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, S glycoprotein, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Host Species: Mouse

Clonality: Monoclonal

Format: IgG

Target Details

Reactivity: SARS-CoV-2

Immunogen Type: Recombinant Protein

Immunogen: This protein A purified antibody was produced by repeated immunizations with a purified SARS-CoV-2 S protein with His-tag, derived from the transfected human HEK293 cells.

Purity/Specificity: Anti-SARS-CoV-2 Spike S2 Antibody is directed against SARS Coronavirus 2 Spike (S2) protein. The product was purified from roller bottle culture by protein A chromatography. BLAST analysis was used to suggest reactivity with related Coronavirus proteins. No reactivity was seen against SARS CoV-2 Spike S1 subunit, SARS CoV-2 Spike S1 RBD, SARS CoV-2 loop, SARS CoV-2 (N), MERS CoV. Cross reactivity with homologues from other sources has not been determined.

Database Links:

P0DTC2 - UniProtKB

QHD43416.1 - NCBI Protein

Application Details

Tested Applications: ELISA

Application Note: Anti-SARS-CoV-2 Spike Protein (S2) Antibody has been tested for use in ELISA and shows reactivity to SARS COV-2 Whole Spike and COV-2 Spike (S2) protein. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 1.12 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein; and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development. Anti-SARS-CoV-2 Spike Protein (S2) Antibody is useful for researchers interested in diagnostics, vaccines, and viral research.

Related Protocols

ELISA Protocol

Sandwich ELISA Protocol for Collagen

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.