Apolipoprotein A-I (Apo A-I)
Référence A2299-30-1mg
Conditionnement : 1mg
Marque : US Biological
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A2299-30 Apolipoprotein A-I (Apo A-I)
Clone Type
PolyclonalGrade
Highly PurifiedShipping Temp
Blue IceStorage Temp
-20°CPreparation of Protein:|Apo AI can be renatured into PBS, TBS, or other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14,000 or less. This should be performed in a cold room.||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |Note: Dialyzed Apo AI in PBS or TBS with 1mM EDTA and 0.1% sodium azide may be stored for up to 2 weeks at 4°C. Storage of Apo AI in PBS or TBS at -20°C may lead to precipitation when thawed.
Applications
Source: High-density lipoprotein (HDL) from pooled human plasma|Purity: ≥99% (SDS-PAGE). Isolated by ultracentrifugal flotation between densities 1.063–1.21g/mLl lyophilized, then subjected to repeated ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6M guanidine hydrochloride and ~25mM DTT. Product was then subjected to Sephacryl S200 filtration and eluted.|Concentration: ~1mg/ml|Form: Supplied as a liquid containing 3M guanidine hydrochloride, 10mM Tris, 100mM sodium chloride, 1mM EDTA and 1mM sodium azide, pH 7.4.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid containing 3M guanidine hydrochloride, 10mM Tris, 100mM sodium chloride, 1mM EDTA and 1mM sodium azide, pH 7.4.
Purity
≥99% (SDS-PAGE). Isolated by ultracentrifugal flotation between densities 1.063–1.21g/mLl lyophilized, then subjected to repeated ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6M guanidine hydrochloride and ~25mM DTT. Product was then subjected to Sephacryl S200 filtration and eluted.