Biological Activity

Aprotinin is a competitive serine protease inhibitor. Aprotinin reversibly binds to and blocks the enzymatic active site. Aprotinin inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin.

Activity: 5868KIU/mg (4.51TIU/mg)

Product Citations

• 

  Cells. 2021 Feb 18;10(2):430.

  Knockdown of AKT3 Activates HER2 and DDR Kinases in Bone-Seeking Breast Cancer Cells, Promotes Metastasis In Vivo and Attenuates the TGFβ/CTGF Axis
  Aprotinin purchased from AbMole

Protocol (for reference only)

Cell Experiment
Cell lines	mouse G8-1 and C2C12 skeletal muscle myoblasts
Preparation method	Olating mouse G8- 1 myoblasts DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reache approximately 40-50% confluence, adding different protease inhibitors to the culture media and incubating cells overnight. Then switching cells to differentiation-promoting media (DMEM + 10% horse serum ?protease inhibitor) and incubating for 7 days.
Concentrations	2 TIU/ml
Incubation time	12hrs

Animal Experiment
Animal models	Male Wistar rats
Formulation	physiological saline
Dosages	1.5 mg/kg and 3 mg/kg/h, 3 mg/kg and 6 mg/kg/h up to 5 mg/kg and 10 mg/kg/h ological saline
Administration	bolus injection followed by a maintenance infusion(i.p.)

Chemical Information

Molecular Weight	6511.48
Formula	C284H432N84O79S7
CAS Number	9087-70-1
Solubility (25°C)	Water ≥ 50 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Reichel CA, et al. PLoS One. Plasmin inhibitors prevent leukocyte accumulation and remodeling events in the postischemic microvasculature.