beta-Hydroxybutyric acid ELISA Kit
Référence OKEH02581
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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Téléphone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | ELISA-Comp | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology beta-Hydroxybutyric acid ELISA Kit (OKEH02581) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-beta-Hydroxybutyric acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated beta-Hydroxybutyric acid and incubated. The beta-Hydroxybutyric acid found in the sample or standards competes with the biotinylated beta-Hydroxybutyric acid for limited binding sites on the immobilized anti-beta-Hydroxybutyric acid antibody. Excess unbound biotinylated beta-Hydroxybutyric acid and sample or standard beta-Hydroxybutyric acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated beta-Hydroxybutyric acid captured in the well and inversely proportional to the amount of beta-Hydroxybutyric acid which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10umol/L | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 104% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.5% (n = 20) Mean Inter-assay CV%: < 7.3% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: BHA Target Name: beta-Hydroxybutyric acid | ||||||||||||||||||||||
| :: | Pubchem: 441 | ||||||||||||||||||||||
| :: | Chemical formula: C4H8O3 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.048umol/L | ||||||||||||||||||||||
| Specificity | Natural and recombinant General beta-Hydroxybutyric acid | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | BHA, beta-Hydroxybutyric acid, BHA, beta-hydroxybutyrate, 3-hydroxybutyric acid |
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| Protein Name | beta-Hydroxybutyric acid |
| Description of Target | In humans, beta-hydroxybutyrate is synthesized in the liver from acetyl-CoA in a reaction catalyzed by the enzyme beta-hydroxybutyrate dehydrogenase and can be used as an energy source by the brain when blood glucose is low. Diabetic patients can have their ketone levels tested via urine or blood to indicate diabetic ketoacidosis. beta-Hydroxybutyric acid (also known as beta-hydroxybutyrate or 3-hydroxybutyric acid) is a ketone body. It is a chiral compound having two enantiomers, D-3-hydroxybutyric acid and L-3-hydroxybutyric acid. Like the other ketone bodies (acetoacetate and acetone), levels of beta-hydroxybutyrate are raised in ketosis. |