beta Tubulin Monoclonal Antibody

Référence E-AB-20033-200

Conditionnement : 200uL

Marque : Elabscience

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Verified Samples Verified Samples in WB: A549, Rat brain, Mouse brain, Chicken lung, Rabbit testis, Sheep muscle
Verified Samples in IHC: Mouse testis
Verified Samples in IF: Hela
Dilution WB 1:5000-1:10000,  IHC 1:100-1:300,  IF 1:100-1:300
Isotype IgG
Host Mouse
Reactivity Human,  Mouse,  Rat,  Monkey,  Chicken,  Dog,  Hamster,  Rabbit,  Sheep,  Insect,  Yeast
Applications WB,  IHC-p,  IF
Clonality Monoclonal
Immunogen Synthetic Peptide
Abbre β-Tubulin
Synonyms CDCBM,  CDCBM1,  CFEOM3,  CFEOM3A,  FEOM3,  M(beta)3,  M(beta)6,  MC1R,  Neuron specific beta III Tubulin,  Neuron-specific class III beta-tubulin,  QccE-11995,  QccE-15186,  TBB3,  TUBB3,  TUBB4,  Tubb 3,  Tubulin,  Tubulin beta 3,  Tubulin beta 3 chain,  Tubulin beta 4,  beta 3 tubulin,  beta-4
Swissprot
Calculated MW 50 kDa
Observed MW 55 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm>cytoskeleton.
Tissue Specificity Expression is primarily restricted to central and peripheral nervous system.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Protein A purification
Research Areas Cancer,  Neuroscience,  Signal Transduction,  Tags and Cell Markers
Clone No. 8B2
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
Cat.No. Product Name Sizes
E-AB-1001 Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1011 Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated) 500μL , 120μL , 60μL
E-AB-1015 Goat Anti-Mouse IgG(H+L)(FITC conjugated) 500μL , 120μL , 60μL
E-AB-1024 Goat Anti-Mouse IgG(H+L)(Biotin conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1059 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated) 500μL , 120μL , 60μL
E-AB-1076 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated) 500μL , 120μL , 60μL
E-AB-1196 HRP-Goat Anti-Mouse IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R217 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) 50mL , 18mL , 6mL , 3mL
E-IR-R220 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) 10mL , 3mL
E-IR-R221 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) 10mL , 3mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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Référence
Description
Cond.
Prix HT
E-AB-20033-120
 120uL 
E-AB-20033-60
 60uL 
E-AB-20033-30
 30uL