Blood Ammonia Colorimetric Assay Kit
Detection principle
Performance characteristics
Synonyms | NH3 |
Sample type | Serum,plasma |
Sensitivity | 0.01 mmol/L |
Detection range | 0.01-2.5 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 45 min |
Precision | Average inter-assay CV: 7.200%Average intra-assay CV: 4.100% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl) |
Storage | 2-8℃ |
Valid period | 12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-2.5 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Human plasma | 1 |
Mouse serum | 1 |
Rat plasma | 1 |
Dog serum | 1 |
Horse serum | 1 |
Note: The diluent is double distilled water or normal saline (0.9% NaCl).
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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NEUROSCIENCE LETTERS (2021) IF: 3.046
Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidencePMID: 34871743
Sample: Serum -
Frontiers in Medicine (2020) IF: 3.9
Helicobacter pylori as an Initiating Factor of Complications in Patients With Cirrhosis: A Single-Center Observational StudySample: Serum