Blood Ammonia Colorimetric Assay Kit

Référence E-BC-K145-M-96T

Conditionnement : 96T

Marque : Elabscience

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Téléphone : +1 850 650 7790

Detection principle

Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution.

Performance characteristics

Synonyms NH3
Sample type Serum,plasma
Sensitivity 0.01 mmol/L
Detection range 0.01-2.5 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 45 min
Precision Average inter-assay CV: 7.200%Average intra-assay CV: 4.100%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-2.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Mouse serum

1

Rat plasma

1

Dog serum

1

Horse serum

1

Note: The diluent is double distilled water or normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

  1. NEUROSCIENCE LETTERS (2021) IF: 3.046
    Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

    DOI: 10.1016/j.neulet.2021.136385

    PMID: 34871743

    Sample: Serum
  2. Frontiers in Medicine (2020) IF: 3.9
    Helicobacter pylori as an Initiating Factor of Complications in Patients With Cirrhosis: A Single-Center Observational Study

    DOI: 10.3389/fmed.2020.00096

    Sample: Serum

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