Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Bradford Protein Colorimetric Assay Kit

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Référence E-BC-K168-M-96T

Conditionnement : 96T

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Marque : Elabscience

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Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Detection Principle	Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.
Synonyms	TP
Sample Type	Serum,Plasma,Animal tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (550-630 nm, optimum wavelength: 595 nm)
Research Area	Amino Acids And Proteins , Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C/room temperature for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period	12 months
Sensitivity	0.046 mg/mL
Detection Range	0.046-0.6 mg/mL
Precision	inter-assay CV: 8.2% \| intra-assay CV: 3.2%
Assay Time	20 min

Cat.No.	Product Name	Sizes
E-BC-K165-M	Biuret Protein Colorimetric Assay Kit	500Assays , 96T
E-BC-K318-M	BCA Protein Colorimetric Assay Kit	500Assays , 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	90-110
Human plasma	90-110
Rabbit serum	90-110
Rat plasma	90-110
Chicken serum	90-110
10% Mouse kidney tissue homogenate	15-20
10% Mouse lung tissue homogenate	15-20
10% Rat spleen tissue homogenate	15-20
10% Rat heart tissue homogenate	15-20
10% Rat liver tissue homogenate	20-25
293T	1
HL-60	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.