Carbonic Anhydrase II antibody (Peroxidase)
Référence orb344883-100ug
Conditionnement : 100ug
Marque : Biorbyt
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Item 1 of 2
Carbonic Anhydrase II antibody (Peroxidase)
Catalog Number: orb344883
| Catalog Number | orb344883 |
|---|---|
| Category | Antibodies |
| Description | Carbonic Anhydrase II antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, IHC, WB |
| Reactivity | Bovine |
| Isotype | IgG |
| Immunogen | Carbonic Anhydrase II [Bovine Erythrocytes] |
| Concentration | 1 mg/mL |
| Dilution range | ELISA: 1:5,000 - 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined. |
| Conjugation | HRP |
| UniProt ID | P00921 |
| NCBI | NP_848667.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-Carbonic Anhydrase II Antibody Peroxid |
| Note | For research use only |
| Application notes | Anti-Carbonic anhydrase II Peroxidase Conjugated Antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot of Rabbit Anti-Carbonic Anhydrase II Peroxidase Conjugated Antibody. Lane 1: 100 ng. Lane 2: 33.3 ng. Lane 3: 11.1 ng. Lane 4: 3.7 ng. Lane 5: 1.23 ng. Secondary Antibody: Anti-Carbonic Anhydrase II HRP 1 µg/mL. Blocking Buffer: orb348644 for 30 min at RT.
ELISA results of purified Rabbit Carbonic Anhydrase II Antibody tested against Carbonic Anhydrase II (erythrocytes). (Green line) The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.