CCRF-CEM-C7 genomic DNA - 5 microgram
Référence 300398GD5
Conditionnement : 5µg
Marque : CLS Cell Lines Service
CCRF-CEM-C7 Cells
General information
Description | The CCRF-CEM-C7 cell line is a clone derived from the parent CCRF-CEM cell line, which itself originates from a human acute lymphoblastic leukemia (ALL) of the T-cell type. This cell line was established from peripheral blood taken from a 4-year-old female patient with ALL. The CCRF-CEM-C7 cell line is extensively used in biomedical research, particularly in studies related to cancer biology, drug screening, and mechanisms of chemotherapy resistance. CCRF-CEM-C7 cells are characterized by their robust growth in vitro and are commonly used to assess the cytotoxicity of anti-cancer compounds. These cells express several key markers of T-cell development and are often utilized to investigate T-cell leukemia pathogenesis, T-cell signaling pathways, and the cellular responses to DNA damage. The line has also been important in studies investigating the role of apoptosis in cancer cells, making it a valuable resource for understanding the mechanisms of programmed cell death in response to therapeutic agents. Given its origin and characteristics, CCRF-CEM-C7 serves as a model system for T-cell acute lymphoblastic leukemia, providing insights into the biological behavior of this malignancy and offering a platform for testing therapeutic strategies targeting cellular pathways specific to T-cell malignancies. |
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Organism | Human |
Tissue | Blood |
Disease | Childhood T acute lymphoblastic leukemia |
Synonyms | CCRF-CEM C7, CCRF/CEM-C7, CEM-C7, CEM C7, CEMC7, CEM clone 7 |
Characteristics
Age | 3Y11M |
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Gender | Female |
Ethnicity | Caucasian |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | CCRF-CEM-C7 (Cytion catalog number 300398) |
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Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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