Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

CD163 (CD163 Antigen, Hemoglobin Scavenger Receptor, M130, M130 Antigen Precursor, Macrophage-associated Antigen, MM130, Scavenger Receptor Cysteine-rich Type 1 Protein M130) (HRP)

Imprimer

Référence C2548-90CA-HRP-100ul

Conditionnement : 100ul

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Marque : US Biological

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Téléphone : +1 850 650 7790

C2548-90CA-HRP CD163 (CD163 Antigen, Hemoglobin Scavenger Receptor, M130, M130 Antigen Precursor, Macrophage-associated Antigen, MM130, Scavenger Receptor Cysteine-rich Type 1 Protein M130) (HRP)

Clone Type

Polyclonal

Host

mouse

Source

porcine

Swiss Prot

Q2VL90

Isotype

IgG1

Grade

Affinity Purified

Applications

IHC IP WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD163, a 120kD cell surface glycoprotein is expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. |Reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner. ||Applications:|Suitable for use in Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications have not been tested.||Recommended Dilutions:|Immunohistochemistry: Frozen sections|Western Blot: Non-reducing conditions |Optimal dilutions to be determined by the researcher. ||Hybridoma: |X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 10B837 (2A10/11)|Host: mouse|Source: porcine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Porcine alveolar macrophages|Specificity: Recognizes porcine CD163 at ~120kD in alveolar macrophage cell lysates under non-reducing conditions.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Porcine alveolar macrophages.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).

Purity

Purified by Protein A affinity chromatography from tissue culture supernatant.

Specificity

Recognizes porcine CD163 at ~120kD in alveolar macrophage cell lysates under non-reducing conditions.

References

1. Sanchez, M. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation. J. Immunol. 162: 5230-5237. 2. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocyte/ macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens 49: 403-413. 3. Yang, P. et al. (2002) Immune cells in the porcine retina: Distribution, characterization and morphological features.|Invest. Opthalmol. Vis. Sci. 43: 1488-1492. 4. Gomez del Moral, M. et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis J. Virol. 73: 2173-2180. 5. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers. Vet. Immunol. Immunopathol. 80: 93-109. 6. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection. Arch. Virol. 148: 2307-2323. 7. Delrue, I. et al. (2010) Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163. BMC Biotechnol. 10: 48. 8. Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver. Stem Cells. 26: 1347-55. 9. Moreno, S.et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2. Vet Res. 41: 76. 10. Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae. Vet Res. 41: 64. 11. Urbieta Caceres, V.H. et al. (2011) Early experimental hypertension preserves the myocardial microvasculature but aggravates cardiac injury distal to chronic coronary artery obstruction. Am J Physiol Heart Circ Physiol. 300: H693-701. 12. Das, P.B. et al. (2010) The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163. J Virol. 84: 1731-40. 13. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.