CD4 antibody [GK1.5] (FITC)
Référence GTX80145-100ug
Conditionnement : 100ug
Marque : GeneTex
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Téléphone : +1 850 650 7790
![Anti-CD4 antibody [GK1.5] (FITC) used in Flow cytometry (FACS). GTX80145](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-CD4 antibody [GK1.5] (FITC) used in Flow cytometry (FACS). GTX80145")
Host | Rat |
|---|---|
Clonality | Monoclonal |
Clone Name | GK1.5 |
Isotype | IgG2b |
Application | FACS |
Reactivity | Mouse, Golden Syrian Hamster |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX80145 on CiteAb
PRODUCT
Summary
The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | Assay dependent |
Not tested in other applications.
Calculated MW
51 kDa. ( Note )
Product Note
This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Mouse CTL clone V4 cells
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Fluorescein isothiocyanate (FITC) Wavelength
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD4 antigen , L3T4 , Ly-4
Cellular Localization
Cell membrane
Database
DATA IMAGES
| | GTX80145 FACS Image FACS analysis of C57Bl/6 splenocytes using GTX80145 CD4 antibody [GK1.5] (FITC). |
Clone Reference
Chi-Shuan Fan et al. Cells 2022; 11 (2) Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization.
Qizhi Ma et al. Precis Clin Med 2021; 4 (3):155-167 CXCL13 expression in mouse 4T1 breast cancer microenvironment elicits antitumor immune response by regulating immune cell infiltration.
2020; In situ vaccination at a peripheral tumor site augments response against melanoma brain metastases
Fu Y et al. Mol Carcinog 2019; Downregulation of CPA4 inhibits non small-cell lung cancer growth by suppressing the AKT/c-MYC pathway.
Huang CP et al. Cytotherapy 2017; (Epub) The anti-tumor effect of intravesical administration of normal urothelial cells on bladder cancer.
Submit a Reference REVIEW
Clone Reference
Chi-Shuan Fan et al. Cells 2022; 11 (2) Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization.
Qizhi Ma et al. Precis Clin Med 2021; 4 (3):155-167 CXCL13 expression in mouse 4T1 breast cancer microenvironment elicits antitumor immune response by regulating immune cell infiltration.
2020; In situ vaccination at a peripheral tumor site augments response against melanoma brain metastases
Fu Y et al. Mol Carcinog 2019; Downregulation of CPA4 inhibits non small-cell lung cancer growth by suppressing the AKT/c-MYC pathway.
Huang CP et al. Cytotherapy 2017; (Epub) The anti-tumor effect of intravesical administration of normal urothelial cells on bladder cancer.
2017; Intestinal microbiota link lymphopenia to murine autoimmunity via PD-1 + CXCR5 -/dim B-helper T cell induction
Das S et al. Proc Natl Acad Sci U S A 2016; 113 (46):13132-13137 GSDMB induces an asthma phenotype characterized by increased airway responsiveness and remodeling without lung inflammation.
2016; Multispectral Imaging of T and B Cells in Murine Spleen and Tumor
Liu YJ et al. PLoS One 2015; 10 (9):e0136214 Dendritic Cells Regulate Treg-Th17 Axis in Obstructive Phase of Bile Duct Injury in Murine Biliary Atresia.
Miller M et al. J Immunol 2014; 192 (8):3475-87 ORMDL3 transgenic mice have increased airway remodeling and airway responsiveness characteristic of asthma.
2014; Polarized axonal surface expression of neuronal KCNQ potassium channels is regulated by calmodulin interaction with KCNQ2 subunit
2012; Subcellular distribution of Lck during CD4 T-cell maturation in the thymic medulla regulates the T-cell activation threshold
2012; Anti-IL-7 receptor-α reverses established type 1 diabetes in nonobese diabetic mice by modulating effector T-cell function
2012; Nab2 regulates secondary CD8+ T-cell responses through control of TRAIL expression
2012; Detection of an autoreactive T-cell population within the polyclonal repertoire that undergoes distinct autoimmune regulator (Aire)-mediated selection
2011; Type I interferon is selectively required by dendritic cells for immune rejection of tumors
2011; T cells are not required for pathogenesis in the Syrian hamster model of hantavirus pulmonary syndrome
2008; Regulated movement of CD4 in and out of the immunological synapse
2007; Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |