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Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd4 monoclonal antibody, clone GK1.5 (FITC) (Cat # MAB5918), gated on small lymphocytes and analyzed by flow cytometry.
- Specifications
Product Description
Rat monoclonal antibody raised against Cd4.
Immunogen
Cloned mouse CTL line V41.
Host
Rat
Reactivity
Mouse
Specificity
CD4/L3T4a, Mr 52 KDa.
Form
Liquid
Conjugation
APC/Cy5.5
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd4 monoclonal antibody, clone GK1.5 (FITC) (Cat # MAB5918), gated on small lymphocytes and analyzed by flow cytometry. - Gene Info — Cd4
- Publication Reference
- Repertoire development and ligand specificity of murine TCR gamma delta cells.
Bluestone JA, Cron RQ, Barrett TA, Houlden B, Sperling AI, Dent A, Hedrick S, Rellahan B, Matis LA.
Immunological Reviews 1991 Apr; 120:5.
- CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus.
Wu L, Scollay R, Egerton M, Pearse M, Spangrude GJ, Shortman K.
Nature 1991 Jan; 349(6304):71.
- Role of L3T4 and Ia in the heteroclitic response of T cells to cytochrome c.
Lakey EK, Margoliash E, Fitch FW, Pierce SK.
Journal of Immunology 1986 Jun; 136(11):3933.
- Repertoire development and ligand specificity of murine TCR gamma delta cells.