CD81 Mouse Monoclonal Antibody (5A6), R-PE Conjugate - Biotium Choice

Note: CD81 clone 5A6 is being discontinued and has been replaced by CD81 Recombinant Monoclonal Mouse Antibody (2304.81) – Biotium Choice, which has been validated to have the same bright and specific staining of human CD81.

CD81 (clone 5A6) is a mouse monoclonal antibody that recognizes the human tetraspanin protein CD81. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. This antibody has been validated in flow cytometry, in immunofluorescence microscopy, and in western blot. The antibody is available conjugated to R-Phycoerythrin (R-PE or PE) or RPE-Astral™616. It is supplied in PBS, 0.1% BSA, 0.05% azide.

CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody. View our full catalog of Biotium Choice antibodies, optimized and validated for the best results in flow cytometry.

Exosome Stains and Antibodies

Biotium also offers ExoBrite™ CD81 Flow Antibody and ExoBrite™ CD81 Western Antibody that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting. Learn more about our products for exosome research.

CD81 Monoclonal Mouse Antibody (5A6) - Biotium Choice